Transformation of dimethyl phthalate esters (DMPEs) by a marine red yeast Rhodotorula mucilaginosa isolated from deep sea sediments of the Atlantic Ocean
Transformation of dimethyl phthalate esters (DMPEs) by a marine red yeast Rhodotorula mucilaginosa isolated from deep sea sediments of the Atlantic Ocean
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从大西洋深海沉积物中分离出的海洋红酵母对邻苯二甲酸二甲酯 (DMPE) 的转化
DOI：
10.1016/j.ibiod.2016.02.006
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发表时间：
2016-04
影响因子：
4.8
通讯作者：
Tian-Ling Zheng
中科院分区：
文献类型：
--
作者：
Jing-Wen Wang;Zhu-Hua Luo;Wei Xu;Jie-Fei Ding;Tian-Ling Zheng
利用富集培养技术从大西洋深海沉积物中分离出一种能够降解邻苯二甲酸二甲酯（DMPE）的海洋红酵母。通过内转录间隔区（ITS）基因序列分析，将该酵母鉴定为Rhodotorula mucilaginosaMar-Y3。使用该酵母研究了三种 DMPE 异构体，即邻苯二甲酸二甲酯 (DMP)、间苯二甲酸二甲酯 (DMI) 和对苯二甲酸二甲酯 (DMT) 的生化降解途径。酵母不能完全矿化 DMPE，但可以将它们转化为相应的邻苯二甲酸酯单酯或邻苯二甲酸。 mucilaginosaMar-Y3 还通过不同的代谢途径和生物降解率降解不同的 DMPE 异构体。酵母以较低的降解率进行一步酯水解，将DMP和DMI转化为各自的单酯；然而，邻苯二甲酸单甲酯（MMP）和间苯二甲酸单甲酯（MMI）是死胡同产物，无法进行进一步的代谢。酵母以较高的降解率进行连续酯水解，通过对苯二甲酸单甲酯(MMT)将DMT转化为对苯二甲酸(TA)，但TA难以进一步代谢。这些结果表明深海酵母R.产生邻苯二甲酸酯酶。 mucilaginosaMar-Y3 对不同的 DMPE 异构体具有非常高的底物特异性，并且该酵母对 DMPE 的降解涉及负责水解两个相同的羧酸酯键的不同酯酶。
A marine red yeast capable of degrading dimethyl phthalate esters (DMPEs) was isolated from deep-sea sediments of the Atlantic Ocean using enrichment culture technique. The yeast was identified asRhodotorula mucilaginosaMar-Y3 based on internal transcribed spacer (ITS) gene sequence analysis. The biochemical degradation pathways of three DMPE isomers, namely dimethyl phthalate (DMP), dimethyl isophthalate (DMI), and dimethyl terephthalate (DMT), were investigated using this yeast. The yeast cannot completely mineralize DMPEs, but can transform them to respective phthalate monoester or phthalic acid.R. mucilaginosaMar-Y3 also degraded different DMPE isomers through varied metabolism pathways and biodegradation rates. The yeast performed one-step ester hydrolysis with a relatively low degradation rate to transform DMP and DMI to the respective monoester; however, monomethyl phthalate (MMP) and monomethyl isophthalate (MMI) were the dead-end products and further metabolism did not proceed. The yeast carried out sequential ester hydrolysis with a relatively high degradation rate to transform DMT to terephthalic acid (TA) via monomethyl terephthalate (MMT), but TA was resistant to further metabolism. These results suggested that phthalate esterases produced by the deep-sea yeastR. mucilaginosaMar-Y3 had a very high substrate specificity for different DMPE isomers, and that the degradation of DMPEs by this yeast involved distinct esterases responsible for the hydrolysis of two identical carboxylic ester bonds.
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影响因子：
5.8
作者：
Zhuhua Luo;Yi-Rui Wu;K. Pang;J. Gu;L. Vrijmoed
通讯作者：
Zhuhua Luo;Yi-Rui Wu;K. Pang;J. Gu;L. Vrijmoed
DOI：
10.1016/j.bbrc.2004.07.089
发表时间：
2004-09
影响因子：
3.1
作者：
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通讯作者：
R. Stingley;Ashraf Khan;C. Cerniglia
影响因子：
5.8
作者：
Zhuhua Luo;K. Pang;J. Gu;R. Chow;L. Vrijmoed
通讯作者：
Zhuhua Luo;K. Pang;J. Gu;R. Chow;L. Vrijmoed
影响因子：
13.6
作者：
S. Yuan;I-Chun Huang;B. Chang
通讯作者：
S. Yuan;I-Chun Huang;B. Chang
影响因子：
4.1
作者：
J. Gartshore;D. Cooper;J. Nicell
通讯作者：
J. Gartshore;D. Cooper;J. Nicell