Fluorogenic sialic acid glycosides for quantification of sialidase activity upon unnatural substrates.
Fluorogenic sialic acid glycosides for quantification of sialidase activity upon unnatural substrates.
复制标题
荧光唾液酸糖苷，用于定量非天然底物上的唾液酸酶活性。
DOI：
10.1016/j.bmcl.2013.03.076
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发表时间：
2013
影响因子：
2.7
通讯作者：
Kumar,Krishna
中科院分区：
文献类型：
--
作者：
Zamora,CristinaY;d'Alarcao,Marc;Kumar,Krishna
Herein we report the synthesis of N-acetyl neuraminic acid derivatives as 4-methylumbelliferyl glycosides and their use in fluorometrically quantifying human and bacterial sialidase activity and substrate specificities. We found that sialidases in the human promyelocytic leukemic cell line HL60 were able to cleave sialic acid substrates with fluorinated C-5 modifications, in some cases to a greater degree than the natural N-acetyl functionality. Human sialidases isoforms were also able to cleave unnatural substrates with bulky and hydrophobic C-5 modifications. In contrast, we found that a bacterial sialidase isolated from Clostridium perfringens to be less tolerant of sialic acid derivatization at this position, with virtually no cleavage of these glycosides observed. From our results, we conclude that human sialidase activity is a significant factor in sialic acid metabolic glycoengineering efforts utilizing unnatural sialic acid derivatives. Our fluorogenic probes have enabled further understanding of the activities and substrate specificities of human sialidases in a cellular context.
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影响因子：
14.8
作者：
Gadhoum, Samah Zeineb;Sackstein, Robert
通讯作者：
Sackstein, Robert
影响因子：
4
作者：
Parker, Randy B.;Kohler, Jennifer J.
通讯作者：
Kohler, Jennifer J.
影响因子：
2.7
作者：
Dafik L;d'Alarcao M;Kumar K
通讯作者：
Kumar K
DOI：
10.1016/j.saa.2011.05.013
发表时间：
2011-09-01
影响因子：
4.4
作者：
Al-Kady, Ahmed S.;Ahmed, El-Sadat I.;Ebeid, El-Zeiny M.
通讯作者：
Ebeid, El-Zeiny M.
影响因子：
4.3
作者：
Sakarya, S;Rifat, S;Goldblum, SE
通讯作者：
Goldblum, SE