Genome-based classification of micromonosporae with a focus on their biotechnological and ecological potential.
Genome-based classification of micromonosporae with a focus on their biotechnological and ecological potential.
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DOI：
10.1038/s41598-017-17392-0
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发表时间：
2018-01-11
影响因子：
4.6
通讯作者：
Goodfellow M
中科院分区：
文献类型：
--
作者：
Carro L;Nouioui I;Sangal V;Meier-Kolthoff JP;Trujillo ME;Montero-Calasanz MDC;Sahin N;Smith DL;Kim KE;Peluso P;Deshpande S;Woyke T;Shapiro N;Kyrpides NC;Klenk HP;Göker M;Goodfellow M
有必要澄清放线菌属小单孢菌，家庭的模式属小单孢菌，鉴于其生物技术和生态的重要性。在这里，通过细菌和真菌基因组百科全书项目获得了40个小单孢菌型菌株和两个非型菌株的基因组草案，并用于生成一个微生物基因组树，该基因组树显示它们可以被分配到支持良好的系统系，这些系统系在基于保守基因的单个和串联序列的相应树中不明显。来自基因组序列的DNA G+C比率表明，来自物种描述的相应数据是不精确的。修订的描述包括精确的碱基组成数据和模式菌株的近似基因组大小。对基因组草案的antiSMASH分析表明，小单孢菌具有以前未意识到的合成新的特化代谢物的潜力。检测到近一千个生物合成基因簇，包括不连续分布的NRPS、PKS、萜烯和铁载体簇，从而开辟了优先选择天然产物发现的优良菌株的前景。关键胁迫相关基因的分布提供了小单孢菌如何适应关键环境变量的见解。与植物相互作用相关的基因突出了小单孢菌在农业和生物技术中的潜在用途。
There is a need to clarify relationships within the actinobacterial genus Micromonospora, the type genus of the family Micromonosporaceae, given its biotechnological and ecological importance. Here, draft genomes of 40 Micromonospora type strains and two non-type strains are made available through the Genomic Encyclopedia of Bacteria and Archaea project and used to generate a phylogenomic tree which showed they could be assigned to well supported phyletic lines that were not evident in corresponding trees based on single and concatenated sequences of conserved genes. DNA G+C ratios derived from genome sequences showed that corresponding data from species descriptions were imprecise. Emended descriptions include precise base composition data and approximate genome sizes of the type strains. antiSMASH analyses of the draft genomes show that micromonosporae have a previously unrealised potential to synthesize novel specialized metabolites. Close to one thousand biosynthetic gene clusters were detected, including NRPS, PKS, terpenes and siderophores clusters that were discontinuously distributed thereby opening up the prospect of prioritising gifted strains for natural product discovery. The distribution of key stress related genes provide an insight into how micromonosporae adapt to key environmental variables. Genes associated with plant interactions highlight the potential use of micromonosporae in agriculture and biotechnology.
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DOI：
10.1007/s10482-013-9886-4
发表时间：
2013-05-01
影响因子：
2.6
作者：
Ahmed, Lina;Jensen, Paul R.;Goodfellow, Michael
通讯作者：
Goodfellow, Michael
DOI：
10.1007/978-1-4939-6634-9_2
发表时间：
2017-01-01
期刊：
ANTIBIOTICS: METHODS AND PROTOCOLS
影响因子：
--
作者：
Adamek, Martina;Spohn, Marius;Ziemert, Nadine
通讯作者：
Ziemert, Nadine
影响因子：
1.2
作者：
Ara, Ismet;Kudo, Takuji
通讯作者：
Kudo, Takuji
影响因子：
56.9
作者：
Béjà, O;Aravind, L;DeLong, EF
通讯作者：
DeLong, EF
影响因子：
7.4
作者：
Aeschbacher, RA;Müller, J;Wiemken, A
通讯作者：
Wiemken, A