Collagen fibers and inflammatory cells in healthy and diseased human gingival tissues: a comparative and quantitative study by immunohistochemistry and automated image analysis.
Collagen fibers and inflammatory cells in healthy and diseased human gingival tissues: a comparative and quantitative study by immunohistochemistry and automated image analysis.
复制标题
健康和患病人类牙龈组织中的胶原纤维和炎症细胞：通过免疫组织化学和自动图像分析进行的比较和定量研究。
DOI：
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Nicole Brousse
中科院分区：
文献类型：
--
作者：
S. Séguier;Gaston Godeau;Nicole Brousse
背景
牙周病的组织学特征是与炎性细胞群的牙龈浸润相关的细胞外基质成分的降解。这项原位研究的目的是量化炎症细胞亚群和面积分数（AA%）所占的胶原纤维在健康和患病的上牙龈结缔组织，以调查之间的关联，如果有的话，胶原损失和炎症细胞浸润。
方法
对来自10名健康对照（C）、9名牙龈炎患者（G）和10名重度慢性牙周炎患者（P）的石蜡牙龈组织切片进行抗CD 45、CD 3、CD 8、CD 20、CD 68、TIA-1和GrB分子的抗体的免疫化学染色，并使用天狼星红F3 Ba对胶原纤维进行染色。通过形态计量学和自动图像分析进行炎性细胞数量和胶原纤维所占AA%的定量评价。
结果
P组CD 45+、CD 20+、CD 68+、TIA-1+、GrB+细胞数较C组、G组均明显增加（P<0.05）。本研究显示C组（63%）、G组（46%）和P组（26%）的AA%的平均值之间存在显著差异（P <0.01），并且G组和P组的AA%与TIA-1+细胞（P <0.01）和GrB+细胞（分别为P <0.01和P<0.05）的数量呈负相关。
结论
这项研究表明，根据牙周病的严重程度，不同的炎症细胞亚群的数量有很大的差异，并表明激活的细胞毒性细胞可以在这些病理状态下观察到的胶原纤维的损失中发挥关键作用。在牙周炎期间，胶原损失与所有炎性细胞亚群数量显著相关。最后，牙龈胶原纤维所占面积分数的定量评价可以反映牙周病的临床严重程度。
BACKGROUND
Periodontal disease is histologically characterized by the degradation of extracellular matrix components associated with a gingival infiltration of inflammatory cell populations. The purpose of this in situ study was to quantify inflammatory cell subsets and the area fraction (AA%) occupied by collagen fibers in healthy and diseased upper gingival connective tissue in order to investigate the association, if any, between collagen loss and inflammatory cell infiltrate.
METHODS
Paraffin gingival tissue sections from 10 healthy controls (C), 9 patients with gingivitis (G), and 10 patients with severe chronic periodontitis (P) were immunohistochemically stained by antibodies against CD45, CD3, CD8, CD20, CD68, TIA-1, and GrB molecules, and the collagen fibers were stained using sirius red F3Ba. The quantitative evaluations of inflammatory cell numbers and the AA% occupied by collagen fibers were performed by morphometric and automated image analysis.
RESULTS
In group P, CD45+, CD20+, CD68+, TIA-1+, and GrB+ cell numbers were significantly increased (P<0.05) when compared to both C and G groups. The present study revealed significant differences (P <0.01) between means of AA% observed in group C (63%), group G (46%), and group P (26%), and AA% of group G and group P was inversely correlated with the numbers of TIA-1+ cells (P<0.01) and GrB+ cells (P<0.01 and P<0.05, respectively).
CONCLUSIONS
This study showed great differences in the number of the distinct inflammatory cell subsets according to the severity of the periodontal disease and suggested that activated cytotoxic cells could play a pivotal role in the loss of collagen fibers observed during these pathological states. During periodontitis, collagen loss was significantly correlated with all inflammatory cell subset numbers. Finally, the quantitative evaluation of the area fraction occupied by gingival collagen fibers may reflect the clinical severity of the periodontal disease.
影响因子：
4.9
作者：
GLETTE, J;SANDBERG, S;SOLBERG, CO
通讯作者：
SOLBERG, CO
影响因子：
4.3
作者：
ALVARES, O;KLEBE, R;COCHRAN, DL
通讯作者：
COCHRAN, DL
影响因子：
3.5
作者：
Taubman,MA;Stoufi,ED;Ebersole,JL;Smith,DJ
通讯作者：
Smith,DJ