Self-Collected Oral Fluid Saliva Is Insensitive Compared With Nasal-Oropharyngeal Swabs in the Detection of Severe Acute Respiratory Syndrome Coronavirus 2 in Outpatients.
Self-Collected Oral Fluid Saliva Is Insensitive Compared With Nasal-Oropharyngeal Swabs in the Detection of Severe Acute Respiratory Syndrome Coronavirus 2 in Outpatients.
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在门诊患者中，与鼻-口咽拭子相比，自采集口腔液唾液检测严重急性呼吸综合征冠状病毒2不敏感。
DOI：
10.1093/ofid/ofaa648
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发表时间：
2021-03
影响因子：
4.2
通讯作者：
Ambulatory COVID Team
中科院分区：
文献类型：
--
作者：
Manabe YC;Reuland C;Yu T;Azamfirei R;Hardick JP;Church T;Brown DM;Sewell TT;Antar A;Blair PW;Heaney CD;Pekosz A;Thomas DL;Ambulatory COVID Team
控制严重急性呼吸综合征冠状病毒2 （SARS-CoV-2）大流行将需要广泛获得准确诊断。唾液取样绕过了拭子供应链瓶颈，易于自我收集，与鼻咽拭子相比，在收集过程中产生气溶胶的可能性更小。我们比较了来自2019冠状病毒病（COVID-19）非住院门诊队列患者多个时间点的匹配唾液唾液液（Oracol设备中收集的牙龈沟液）和鼻-口咽（OP）自采标本的实时逆转录聚合酶链反应雅培m2000结果。这两句话应该放在结果的开头。共有171对匹配的标本。与鼻拭子相比，41.6%的口腔液标本呈阳性。将唾液添加到口腔液百分比收集装置中，将阳性同意率从37.2%（43人中16人）提高到44.6%（65人中29人）。在出现症状后的前5天，阳性同意率最高，此后下降。所有传染性鼻- op样本（VeroE6 TMPRSS2细胞培养阳性）均具有匹配的SARS-CoV-2阳性口腔液样本。在这项对非住院sars - cov -2感染者的研究中，我们发现，与鼻腔- op标本相比，自采口腔液的诊断敏感性较低，这种差异在症状出现后5天以上尤为突出。这些数据并不能证明常规使用口腔液体采集来诊断SARS-CoV-2是合理的，尽管收集起来更容易。它还强调了考虑唾液标本采集方法和时间从症状发作的重要性，特别是在门诊人群。在一项对未住院的COVID-19患者的研究中，口腔液体唾液样本类型与鼻- op样本类型相比总体不敏感，但随着唾液的加入而改善，并且在症状出现后的前5天最敏感。
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic control will require widespread access to accurate diagnostics. Salivary sampling circumvents swab supply chain bottlenecks, is amenable to self-collection, and is less likely to create an aerosol during collection compared with the nasopharyngeal swab. We compared real-time reverse-transcription polymerase chain reaction Abbott m2000 results from matched salivary oral fluid (gingival crevicular fluid collected in an Oracol device) and nasal-oropharyngeal (OP) self-collected specimens in viral transport media from a nonhospitalized, ambulatory cohort of coronavirus disease 2019 (COVID-19) patients at multiple time points. These 2 sentences should be at the beginning of the results. There were 171 matched specimen pairs. Compared with nasal-OP swabs, 41.6% of the oral fluid samples were positive. Adding spit to the oral fluid percent collection device increased the percent positive agreement from 37.2% (16 of 43) to 44.6% (29 of 65). The positive percent agreement was highest in the first 5 days after symptoms and decreased thereafter. All of the infectious nasal-OP samples (culture positive on VeroE6 TMPRSS2 cells) had a matched SARS-CoV-2 positive oral fluid sample. In this study of nonhospitalized SARS-CoV-2-infected persons, we demonstrate lower diagnostic sensitivity of self-collected oral fluid compared with nasal-OP specimens, a difference that was especially prominent more than 5 days from symptom onset. These data do not justify the routine use of oral fluid collection for diagnosis of SARS-CoV-2 despite the greater ease of collection. It also underscores the importance of considering the method of saliva specimen collection and the time from symptom onset especially in outpatient populations. In a study of nonhospitalized COVID-19 patients, the oral fluid salivary sample type was insensitive compared to nasal-OP specimen types overall, but was improved with the addition of spit and most sensitive in the first 5 days after symptom onset.
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影响因子：
7.6
作者：
Gupta S;Mohindra R;Chauhan PK;Singla V;Goyal K;Sahni V;Gaur R;Verma DK;Ghosh A;Soni RK;Suri V;Bhalla A;Singh MP
通讯作者：
Singh MP
影响因子：
168.9
作者：
Huang, Chaolin;Wang, Yeming;Cao, Bin
通讯作者：
Cao, Bin
DOI：
10.1016/j.ijid.2020.12.080
发表时间：
2021-03
期刊：
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
影响因子：
--
作者：
Ku CW;Shivani D;Kwan JQT;Loy SL;Erwin C;Ko KKK;Ng XW;Oon L;Thoon KC;Kalimuddin S;Chan JKY
通讯作者：
Chan JKY
影响因子：
8.8
作者：
Landry, Marie L.;Criscuolo, Jody;Peaper, David R.
通讯作者：
Peaper, David R.
影响因子：
9.4
作者：
Barat B;Das S;De Giorgi V;Henderson DK;Kopka S;Lau AF;Miller T;Moriarty T;Palmore TN;Sawney S;Spalding C;Tanjutco P;Wortmann G;Zelazny AM;Frank KM
通讯作者：
Frank KM