SARS-CoV-2 Detection in Gingival Crevicular Fluid.
SARS-CoV-2 Detection in Gingival Crevicular Fluid.
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DOI：
10.1177/0022034520970536
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发表时间：
2021-03
影响因子：
7.6
通讯作者：
Singh MP
中科院分区：
文献类型：
--
作者：
Gupta S;Mohindra R;Chauhan PK;Singla V;Goyal K;Sahni V;Gaur R;Verma DK;Ghosh A;Soni RK;Suri V;Bhalla A;Singh MP
了解 2019 年冠状病毒病 (COVID-19) 感染的病理生理学仍然是我们这个时代的重大挑战。龈沟液代表全身状态，并具有检测病毒和生物标志物的良好记录，构成了评估严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 存在的逻辑基础。该研究旨在评估 33 名经鼻咽取样被视为 COVID-19 阳性患者的龈沟液 (GCF) 中是否存在 SARS-CoV-2 证据。还尝试将其与唾液作为 SARS-CoV-2 诊断液的敏感性进行比较评估。 GCF 和唾液样本是从 33 名 COVID-19 确诊患者身上采集的。使用 NucliSENS easyMAG (bioMérieux) 提取总 RNA 并在洗脱缓冲液中洗脱。在 Applied Biosystems 7500 实时机器中使用 TRUPCR SARS-CoV-2 RT qPCR 试剂盒 V-2.0 (I) 检测 GCF 样本中 SARS-CoV-2 的包膜基因（E 基因）和作为内参的人 RNase P 基因。绝大多数无症状和轻度症状患者的 GCF 样本中都表现出新型冠状病毒的存在。考虑到鼻咽拭子采样中存在 SARS-CoV-2 RNA 作为金标准，GCF 和唾液的敏感性分别为 63.64%（置信区间 [CI]，45.1% 至 79.60%）和 64.52%（CI，45.37% 至 80.77%）。研究发现 GCF 检测 SARS-CoV-2 的敏感性与唾液相当。在 12 名 GCF 检测呈阴性的患者中，有 3 名患者的唾液样本检测呈阳性，同样，在实时逆转录聚合酶链式反应唾液检测呈阴性的 11 名患者中，有 2 名 GCF 检测呈阳性。结果确定 GCF 是 SARS-CoV-2 的一种可能传播方式，这是文献中第一份此类报告，并且还提供了第一个可量化的证据，表明 COVID-19 感染与口腔健康之间存在联系。
Understanding the pathophysiology of the coronavirus disease 2019 (COVID-19) infection remains a significant challenge of our times. The gingival crevicular fluid being representative of systemic status and having a proven track record of detecting viruses and biomarkers forms a logical basis for evaluating the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The study aimed to assess gingival crevicular fluid (GCF) for evidence of SARS-CoV-2 in 33 patients who were deemed to be COVID-19 positive upon nasopharyngeal sampling. An attempt was also made to comparatively evaluate it with saliva in terms of its sensitivity, as a diagnostic fluid for SARS-CoV-2. GCF and saliva samples were collected from 33 COVID-19–confirmed patients. Total RNA was extracted using NucliSENS easyMAG (bioMérieux) and eluted in the elution buffer. Envelope gene (E gene) of SARS-CoV-2 and human RNase P gene as internal control were detected in GCF samples by using the TRUPCR SARS-CoV-2 RT qPCR kit V-2.0 (I) in an Applied Biosystems 7500 real-time machine. A significant majority of both asymptomatic and mildly symptomatic patients exhibited the presence of the novel coronavirus in their GCF samples. Considering the presence of SARS-CoV-2 RNA in the nasopharyngeal swab sampling as gold standard, the sensitivity of GCF and saliva, respectively, was 63.64% (confidence interval [CI], 45.1% to 79.60%) and 64.52% (CI, 45.37% to 80.77%). GCF was found to be comparable to saliva in terms of its sensitivity to detect SARS-CoV-2. Saliva samples tested positive in 3 of the 12 patients whose GCF tested negative, and likewise GCF tested positive for 2 of the 11 patients whose saliva tested negative on real-time reverse transcription polymerase chain reaction. The results establish GCF as a possible mode of transmission of SARS-CoV-2, which is the first such report in the literature, and also provide the first quantifiable evidence pointing toward a link between the COVID-19 infection and oral health.
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