Integrative Analyses of mRNA Expression Profile Reveal SOCS2 and CISH Play Important Roles in GHR Mutation-Induced Excessive Abdominal Fat Deposition in the Sex-Linked Dwarf Chicken.
Integrative Analyses of mRNA Expression Profile Reveal SOCS2 and CISH Play Important Roles in GHR Mutation-Induced Excessive Abdominal Fat Deposition in the Sex-Linked Dwarf Chicken.
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mRNA 表达谱的综合分析揭示 SOCS2 和 CISH 在 GHR 突变引起的伴性侏儒鸡腹部脂肪过度沉积中发挥重要作用
DOI：
10.3389/fgene.2020.610605
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发表时间：
2020
影响因子：
3.7
通讯作者：
Luo W
中科院分区：
文献类型：
--
作者：
Chen G;Chen J;Wu J;Ren X;Li L;Lu S;Cheng T;Tan L;Liu M;Luo Q;Liang S;Nie Q;Zhang X;Luo W
Sex-linked dwarf (SLD) chicken, which is caused by a recessive mutation of the growth hormone receptor (GHR), has been widely used in the Chinese broiler industry. However, it has been found that the SLD chicken has more abdominal fat deposition than normal chicken. Excessive fat deposition not only reduced the carcass quality of the broilers but also reduced the immunity of broilers to diseases. To find out the key genes and the precise regulatory pathways that were involved in the GHR mutation-induced excessive fat deposition, we used high-fat diet (HFD) and normal diet to feed the SLD chicken and normal chicken and analyzed the differentially expressed genes (DEGs) among the four groups. Results showed that the SLD chicken had more abdominal fat deposition and larger adipocytes size than normal chicken and HFD can promote abdominal fat deposition and induce adipocyte hypertrophy. RNA sequencing results of the livers and abdominal fats from the above chickens revealed that many DEGs between the SLD and normal chickens were enriched in fat metabolic pathways, such as peroxisome proliferator-activated receptor (PPAR) signaling, extracellular matrix (ECM)-receptor pathway, and fatty acid metabolism. Importantly, by constructing and analyzing the GHR-downstream regulatory network, we found that suppressor of cytokine signaling 2 (SOCS2) and cytokine-inducible SH2-containing protein (CISH) may involve in the GHR mutation-induced abdominal fat deposition in chicken. The ectopic expression of SOCS2 and CISH in liver-related cell line leghorn strain M chicken hepatoma (LMH) cell and immortalized chicken preadipocytes (ICP) revealed that these two genes can regulate fatty acid metabolism, adipocyte differentiation, and lipid droplet accumulation. Notably, overexpression of SOCS2 and CISH can rescue the hyperactive lipid metabolism and excessive lipid droplet accumulation of primary liver cell and preadipocytes that were isolated from the SLD chicken. This study found some genes and pathways involved in abdominal fat deposition of the SLD chicken and reveals that SOCS2 and CISH are two key genes involved in the GHR mutation-induced excessive fat deposition of the SLD chicken.
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DOI：
10.1073/pnas.1717065115
发表时间：
2018-02-13
影响因子：
11.1
作者：
Fang, Yimin;Hill, Cristal M.;Bartke, Andrzej
通讯作者：
Bartke, Andrzej
影响因子：
4.3
作者：
Ginsberg, Shira;Laron, Zvi;Vaisman, Nachum
通讯作者：
Vaisman, Nachum
影响因子：
7.1
作者：
Holloway, Cameron J.;Cochlin, Lowri E.;Clarke, Kieran
通讯作者：
Clarke, Kieran
影响因子：
8.1
作者：
Csaki, Lauren S.;Dwyer, Jennifer R.;Reue, Karen
通讯作者：
Reue, Karen
影响因子：
5.2
作者：
Dehkhoda F;Lee CMM;Medina J;Brooks AJ
通讯作者：
Brooks AJ