In situ characterizing membrane lipid phenotype of breast cancer cells using mass spectrometry profiling.
In situ characterizing membrane lipid phenotype of breast cancer cells using mass spectrometry profiling.
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DOI：
10.1038/srep11298
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发表时间：
2015-06-10
影响因子：
4.6
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
He M;Guo S;Li Z
细胞膜脂质组成与细胞特性密切相关。在此，采用基质辅助激光解吸/电离-傅里叶变换离子回旋共振质谱法原位测定人乳腺上皮细胞（MCF-10 A）和六种不同的乳腺癌细胞系（即，BT-20、MCF-7、SK-BR-3、MDA-MB-231、MDA-MB-157和MDA-MB-361）。偏最小二乘判别分析表明，这些膜脂水平的变化与乳腺细胞系的类型密切相关。检测到MCF-10 A细胞中相对于6种乳腺癌细胞和BT-20细胞中相对于其他乳腺癌细胞系的多不饱和脂质水平升高。Western印迹分析表明，五种脂肪生成相关酶（即，脂肪酸合酶1（FASN 1）、硬脂酰辅酶A去饱和酶1（SCD 1）、硬脂酰辅酶A去饱和酶5（SCD 5）、胆碱激酶α（CKα）和鞘磷脂合酶1）与乳腺细胞的类型相关，并且MCF-7细胞中的SCD 1水平相对于其它乳腺细胞系显著增加。我们的研究结果表明，FASN 1，SCD 1，SCD 5和CKα的表达水平升高可能与乳腺癌细胞系中饱和和单不饱和脂质水平升高密切相关。
Lipid composition in cell membrane is closely associated with cell characteristics. Here, matrix-assisted laser desorption/ionization- Fourier transform ion cyclotron resonance mass spectrometry was employed to in situ determine membrane components of human mammary epithelial cells (MCF-10 A) and six different breast cancer cell lines (i.e., BT-20, MCF-7, SK-BR-3, MDA-MB-231, MDA-MB-157, and MDA-MB-361) without any lipid extraction and separation. Partial least-square discriminant analysis indicated that changes in the levels of these membrane lipids were closely correlated with the types of breast cell lines. Elevated levels of polyunsaturated lipids in MCF-10 A cells relative to six breast cancer cells and in BT-20 cells relative to other breast cancer cell lines were detected. The Western blotting assays indicated that the expression of five lipogenesis-related enzymes (i.e., fatty acid synthase 1(FASN1), stearoyl-CoA desaturase 1 (SCD1), stearoyl-CoA desaturase 5 (SCD5), choline kinase α (CKα), and sphingomyelin synthase 1) was associated with the types of the breast cells, and that the SCD1 level in MCF-7 cells was significantly increased relative to other breast cell lines. Our findings suggest that elevated expression levels of FASN1, SCD1, SCD5, and CKα may closely correlated with enhanced levels of saturated and monounsaturated lipids in breast cancer cell lines.
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