POP-1 controls axis formation during early gonadogenesis in C. elegans.
POP-1 controls axis formation during early gonadogenesis in C. elegans.
复制标题
POP-1 控制线虫早期性腺发生过程中轴的形成。
作者：
K. Siegfried;J. Kimble
线虫性腺的形状和极性是在性腺发生早期由两个体细胞性腺前体细胞Z1和Z4及其后代决定的。Z1和Z4不对称分裂，以建立性腺的近-远端轴，并产生控制器官形状的调节领导细胞。在本文中，我们报道了线虫Tcf/Lef-1转录因子POP-1控制了第一次Z1/Z4不对称分裂，从而控制了近-远端轴的形成。我们已经确定了两个POP-1(Sys)等位基因(对于对称姐妹)，它们使得Z1/Z4分裂对称。POP-1(Q645)等位基因对雌雄同体的Sys性腺发生缺陷是完全穿透的，但对男性性腺的影响很弱；POP-1(Q645)改变了β-连环蛋白结合区的保守氨基酸。POP-1(Q624)等位基因对多种缺陷具有弱穿透性，表现为部分功能缺失突变；POP-1(Q624)改变了HMG-box DNA结合区的保守氨基酸。合子POP-1(RNAi)证实了POP-1在Z1/Z4不对称中的作用，并揭示了POP-1的其他作用，包括在领导细胞迁移中的作用。另外两个Wnt途径调节剂WRM-1和LIT-1对Z1/Z4不对称具有与POP-1相同的作用。因此，WRM-1和LIT-1是POP-1功能所必需的，而不是像在早期胚胎中观察到的那样相反。我们的结论是，POP-1控制Z1/Z4的不对称分裂，从而建立性腺的近-远端轴。这种对近端-远端极性的控制扩展了我们对线虫Wnt信号的看法，此前已知Wnt信号控制着前后极性。
The shape and polarity of the C. elegans gonad is defined during early gonadogenesis by two somatic gonadal precursor cells, Z1 and Z4, and their descendants. Z1 and Z4 divide asymmetrically to establish the proximal-distal axes of the gonad and to generate regulatory leader cells that control organ shape. In this paper, we report that pop-1, the C. elegans TCF/LEF-1 transcription factor, controls the first Z1/Z4 asymmetric division and hence controls proximal-distal axis formation. We have identified two pop-1(Sys) alleles (for symmetrical sisters) that render the Z1/Z4 divisions symmetrical. The pop-1(q645) allele is fully penetrant for the Sys gonadogenesis defect in hermaphrodites, but affects male gonads weakly; pop-1(q645) alters a conserved amino acid in the beta-catenin binding domain. The pop-1(q624) allele is weakly penetrant for multiple defects and appears to be a partial loss-of-function mutation; pop-1(q624) alters a conserved amino acid in the HMG-box DNA binding domain. Zygotic pop-1(RNAi) confirms the role of pop-1 in Z1/Z4 asymmetry and reveals additional roles of pop-1, including one in leader cell migration. Two other Wnt pathway regulators, wrm-1 and lit-1, have the same effect as pop-1 on Z1/Z4 asymmetry. Therefore, wrm-1 and lit-1 are required for pop-1 function, rather than opposing it as observed in the early embryo. We conclude that POP-1 controls the Z1/Z4 asymmetric division and thereby establishes the proximal-distal axes of the gonad. This control over proximal-distal polarity extends our view of Wnt signaling in C. elegans, which had previously been known to control anterior-posterior polarities.
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影响因子：
--
作者：
R. Porter
通讯作者：
R. Porter
多重调控水平指定了秀丽隐杆线虫不对称细胞分裂的极性。
DOI：
10.1242/dev.127.21.4587
发表时间：
2000
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Whangbo,J;Harris,J;Kenyon,C
通讯作者：
Kenyon,C
DOI：
10.1073/pnas.96.11.6048
发表时间：
1999-05-25
影响因子：
11.1
作者：
Morse, DP;Bass, BL
通讯作者：
Bass, BL
影响因子：
4.6
作者：
Michael A. Herman
通讯作者：
Michael A. Herman
影响因子：
2.7
作者：
Paul W. Sternberg;Horvitz Hr
通讯作者：
Paul W. Sternberg;Horvitz Hr