An Extended Approach to Quantify Triacylglycerol in Microalgae by Characteristic Fatty Acids.
An Extended Approach to Quantify Triacylglycerol in Microalgae by Characteristic Fatty Acids.
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DOI：
10.3389/fpls.2017.01949
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发表时间：
2017
影响因子：
5.6
通讯作者：
Chi ZY
中科院分区：
文献类型：
--
作者：
Yang M;Fan Y;Wu PC;Chu YD;Shen PL;Xue S;Chi ZY
微藻是第三代生物燃料原料，因为它们在恶劣的环境条件下三酰甘油(TAG)含量很高。微藻标签存在于单个细胞中，是一种成分复杂的脂类。我们先前的研究表明，TAG具有用于定量的特征脂肪酸(CFA)，并且在一定范围内与微藻中CFA的相对丰度呈线性相关。在此，我们定义了TAG与CFA的线性相关性在含油性微藻莱茵衣藻和三角褐指藻中的应用范围。此外，TAG的定量被进一步扩展到更广泛的水平，验证了在整个氮素胁迫期间，饱和或单不饱和CFAs的绝对量，即莱茵哈迪尔乳杆菌的16：0和18：1n9，三角毛平革菌的16：0和16：1n7，而不是多不饱和的CFAs的绝对量与TAG水平呈线性相关。该方法利用单一脂肪酸对标签混合物进行定量，具有快速、简单和准确的特点，为监测不同微藻物种的标签积累和促进微藻的高通量突变筛选提供了有用的工具。
Microalgae represent a third generation biofuel feedstock due to their high triacylglycerol (TAG) content under adverse environmental conditions. Microalgal TAG resides in a single cell and serves as a lipid class mixed with complicated compositions. We previously showed that TAG possessed characteristic fatty acids (CFAs) for quantification and was linearly correlated with the relative abundance of CFA within certain limits in microalgae. Here, we defined the application range of the linear correlation between TAG and CFA in the oleaginous microalgae Chlamydomonas reinhardtii and Phaeodactylum tricornutum. In addition, TAG quantification was further expanded to a wide range of levels and the absolute amounts of saturated or monounsaturated CFAs, 16:0 and 18:1n9 of C. reinhardtii and 16:0 and 16:1n7 of P. tricornutum, instead of polyunsaturated CFAs, were verified to be linearly correlated to TAG levels throughout the entire period of nitrogen stress. This approach utilizes a single fatty acid to quantify TAG mixtures, and is rapid, simple and precise, which provides a useful tool for monitoring TAG accumulation of distinct microalgal species and facilitating high-throughput mutant screening for microalgae.
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影响因子：
16.6
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1016/j.algal.2014.08.001
发表时间：
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影响因子：
5.1
作者：
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影响因子：
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作者：
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影响因子：
11.4
作者：
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通讯作者：
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