Disordered expression of inhibitory receptors on the NK1‐type natural killer (NK) leukaemic cells from patients with hypersensitivity to mosquito bites
Disordered expression of inhibitory receptors on the NK1‐type natural killer (NK) leukaemic cells from patients with hypersensitivity to mosquito bites
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对蚊虫叮咬过敏的患者的 NK1 型自然杀伤 (NK) 白血病细胞上抑制性受体表达紊乱
DOI：
10.1046/j.1365-2249.2000.01253.x
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发表时间：
2000
影响因子：
4.6
通讯作者：
Masahiro Takigawa
中科院分区：
文献类型：
--
作者：
Naohiro Seo;Y. Tokura;S. Ishihara;Yasunobu Takeoka;S. Tagawa;Masahiro Takigawa
最近的研究发现存在一种独特类型的少年型NK细胞白血病，其主要临床表现为对蚊虫叮咬的超敏反应（HMB），并感染克隆eb病毒（EBV）。因此，这种疾病被称为HMB - EBV - NK病，并在东方人中有报道，主要来自日本。我们研究了三例HMB - EBV - NK疾病患者NK白血病细胞中细胞因子的产生和两种类型NK抑制受体（即CD94凝集素样二聚体和杀伤细胞免疫球蛋白样受体）的表达。研究发现，新分离的NK白血病细胞表达干扰素γ (IFN‐γ) mRNA，并在IL‐2刺激下额外产生IL‐10，表明NK细胞为NK1型。超过98%的NK细胞的CD94水平高于正常NK细胞，而属于免疫球蛋白样受体的p70或NKAT2在这些NK细胞中不表达。新分离的白血病NK细胞以异常高的水平转录CD94相关分子NKG2C的mRNA，并且在IL - 2和/或IL - 12的刺激下，它们也表达NKG2A。这些抑制受体的紊乱表达不仅为HMB - EBV - NK疾病的发病机制提供了一些见解，而且可以作为诊断这种类型NK细胞白血病的表型标记物。
Recent studies have revealed the existence of a distinct type of NK cell leukaemia of the juvenile type, which presents with hypersensitivity to mosquito bites (HMB) as an essential clinical manifestation and is infected with clonal Epstein–Barr virus (EBV). This disorder is thus called HMB‐EBV‐NK disease and has been reported in Orientals, mostly from Japan. We investigated the profile of cytokine production and the expression of both types of NK inhibitory receptors, i.e. CD94 lectin‐like dimers and killer‐cell immunoglobulin‐like receptors, in NK leukaemic cells from three patients with HMB‐EBV‐NK disease. It was found that freshly isolated NK leukaemic cells expressed mRNA for interferon‐gamma (IFN‐γ) and additionally produced IL‐10 upon stimulation with IL‐2, indicating that the NK cells were of NK1 type. More than 98% of NK cells from the patients bore CD94 at a higher level than did normal NK cells, whereas p70 or NKAT2, belonging to immunoglobulin‐like receptor, was not expressed in those NK cells. Freshly isolated leukaemic NK cells transcribed mRNA for CD94‐associated molecule NKG2C at an abnormally high level, and upon stimulation with IL‐2 and/or IL‐12 they expressed NKG2A as well. The disordered expression of these inhibitory receptors not only provides some insights into the pathogenesis of HMB‐EBV‐NK disease but also can be used as phenotypic markers for the diagnosis of this type of NK cell leukaemia.
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编码新型 HLA I 类基因的 cDNA 克隆的分离和核苷酸序列。
DOI：
--
发表时间：
1988
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Mizuno,S;Trapani,JA;Koller,BH;Dupont,B;Yang,SY
通讯作者：
Yang,SY
DOI：
--
发表时间：
1988
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Koller,BH;Geraghty,DE;Shimizu,Y;DeMars,R;Orr,HT
通讯作者：
Orr,HT
影响因子：
4.4
作者：
David Peritt;Susan J. Robertson;G. Gri;Louise C. Showe;M. Aste‐Amezaga;Giorgio Trinchieri
通讯作者：
David Peritt;Susan J. Robertson;G. Gri;Louise C. Showe;M. Aste‐Amezaga;Giorgio Trinchieri
DOI：
10.1073/pnas.95.9.5199
发表时间：
1998-04-28
影响因子：
11.1
作者：
Lee, N;Llano, M;Geraghty, DE
通讯作者：
Geraghty, DE
DOI：
10.1016/0167-5699(96)80616-5
发表时间：
1996-04-01
期刊：
IMMUNOLOGY TODAY
影响因子：
--
作者：
Kos, FJ;Engleman, EG
通讯作者：
Engleman, EG