Encapsulation of Primary Salivary Gland Acinar Cell Clusters and Intercalated Ducts (AIDUCs) within Matrix Metalloproteinase (MMP)-Degradable Hydrogels to Maintain Tissue Structure and Function.
Encapsulation of Primary Salivary Gland Acinar Cell Clusters and Intercalated Ducts (AIDUCs) within Matrix Metalloproteinase (MMP)-Degradable Hydrogels to Maintain Tissue Structure and Function.
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DOI：
10.1002/adhm.202101948
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发表时间：
2022-04
影响因子：
10
通讯作者：
Benoit DSW
中科院分区：
文献类型：
--
作者：
Song Y;Sharipol A;Uchida H;Ingalls MH;Piraino L;Mereness JA;Moyston T;DeLouise LA;Ovitt CE;Benoit DSW
唾液腺细胞（SGCs）在体外的分泌功能维持不佳，限制了唾液腺再生策略的发展。为了减少分泌功能的急剧丧失，研究了分离完整腺泡细胞簇和闰管（AIDUCs）而不是常用的单细胞悬液的改良方法。这种分离方法产生了AIDUC，其保持了完整腺体的许多细胞-细胞和细胞-基质相互作用。包封AIDUCs在基质金属蛋白酶（MMP）可降解的PEG水凝胶促进自组装成唾液腺模拟物（SGm）与腺泡样结构。Mist 1是一种与分泌功能相关的转录因子，在整个体外培养期间长达14天均可检测到其表达。免疫组织化学还证实了腺泡细胞标志物（NKCC 1、PIP和AQP 5）、导管细胞标志物（K7和K5）和肌上皮细胞标志物（SMA）的表达。强大的卡巴胆碱和ATP刺激的钙流量内观察到的SGM长达14天后封装，表明分泌功能保持。虽然在SGm内观察到一些腺泡-导管化生，但与以前的报告相比有所减少。总之，AIDUCs内维持的细胞-细胞相互作用以及水凝胶微环境可能是唾液腺再生策略的有前途的平台。
Progress in the development of salivary gland regenerative strategies is limited by poor maintenance of the secretory function of salivary gland cells (SGCs) in vitro. To reduce the precipitous loss of secretory function, a modified approach to isolate intact acinar cell clusters and intercalated ducts (AIDUCs), rather than commonly used single cell suspension, was investigated. This isolation approach yielded AIDUCs that maintain many of the cell-cell and cell-matrix interactions of intact glands. Encapsulation of AIDUCs in matrix metalloproteinase (MMP)-degradable PEG hydrogels promoted self-assembly into salivary gland mimetics (SGm) with acinar-like structure. Expression of Mist1, a transcription factor associated with secretory function, was detectable throughout the in vitro culture period up to 14 days. Immunohistochemistry also confirmed expression of acinar cell markers (NKCC1, PIP and AQP5), duct cell markers (K7 and K5), and myoepithelial cell markers (SMA). Robust carbachol and ATP-stimulated calcium flux was observed within the SGm for up to 14 days after encapsulation, indicating that secretory function is maintained. Though some acinar-to-ductal metaplasia was observed within SGm, it was reduced compared to previous reports. In conclusion, cell-cell interactions maintained within AIDUCs together with the hydrogel microenvironment may be a promising platform for salivary gland regenerative strategies.
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影响因子：
3
作者：
Varghese JJ;Hansen ME;Sharipol A;Ingalls MH;Ormanoski MA;Newlands SD;Ovitt CE;Benoit DSW
通讯作者：
Benoit DSW
影响因子：
3.7
作者：
An H;Kim JY;Oh E;Lee N;Cho Y;Seo JH
通讯作者：
Seo JH
影响因子：
9.7
作者：
Hoffman, Michael D.;Van Hove, Amy H.;Benoit, Danielle S. W.
通讯作者：
Benoit, Danielle S. W.
影响因子：
2.8
作者：
CASELITZ, J;SCHMITT, P;SCHUPPAN, D
通讯作者：
SCHUPPAN, D
DOI：
10.1097/01.wcb.0000141558.40491.75
发表时间：
2004-12-01
影响因子：
6.3
作者：
Hedtjarn, M;Mallard, C;Hagberg, H
通讯作者：
Hagberg, H