Salivary gland cell aggregates are derived from self-organization of acinar lineage cells.
Salivary gland cell aggregates are derived from self-organization of acinar lineage cells.
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DOI：
10.1016/j.archoralbio.2018.10.017
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发表时间：
2019-01
影响因子：
3
通讯作者：
Benoit DSW
中科院分区：
文献类型：
--
作者：
Varghese JJ;Hansen ME;Sharipol A;Ingalls MH;Ormanoski MA;Newlands SD;Ovitt CE;Benoit DSW
本研究的目的是表征唾液腺细胞（SGC）在体外聚集的机制。使用原代鼠和人唾液腺细胞，利用延时显微镜分析唾液腺聚集体形成的过程。研究了细胞密度、增殖、细胞外钙和分泌腺泡细胞在聚集体形成中的作用。最后，还评价了从辐照腺体分离的细胞形成聚集体的能力。唾液腺细胞的自组织，而不是增殖是主要的机制，在这两个主要的小鼠和人类唾液腺文化（SGC）的聚集体形成。发现聚集需要细胞外钙，而腺泡谱系细胞占总聚集细胞群的约80%。最后，聚集不受辐射损害。这些数据表明，聚集发生作为异质唾液腺细胞自组织的结果，而不是从干细胞增殖和分化，与以前的教条相矛盾。这些结果表明，重新评估的聚集体形成的标准定义唾液腺干细胞。
The objective of this study was to characterize the mechanism by which salivary gland cells (SGC) aggregate in vitro. Timelapse microscopy was utilized to analyze the process of salivary gland aggregate formation using both primary murine and human salivary gland cells. The role of cell density, proliferation, extracellular calcium, and secretory acinar cells in aggregate formation was investigated. Finally, the ability of cells isolated from irradiated glands to form aggregates was also evaluated. Salivary gland cell self-organization rather than proliferation was the predominant mechanism of aggregate formation in both primary mouse and human salivary gland cultures (SGC). Aggregation was found to require extracellular calcium while acinar lineage cells account for ~80% of the total aggregate cell population. Finally, aggregation was not impaired by irradiation. The data reveal that aggregation occurs as a result of heterogeneous salivary gland cell self-organization rather than from stem cell proliferation and differentiation, contradicting previous dogma. These results suggest a re-evaluation of aggregate formation as a criterion defining salivary gland stem cells.
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影响因子：
3.7
作者：
Chen SF;Chang YC;Nieh S;Liu CL;Yang CY;Lin YS
通讯作者：
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影响因子：
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影响因子：
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作者：
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通讯作者：
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