Expression of telomerase activity in human endometrium is localized to epithelial glandular cells and regulated in a menstrual phase-dependent manner correlated with cell proliferation.-猫眼课题宝

人子宫内膜中端粒酶活性的表达定位于上皮腺细胞，并以与细胞增殖相关的月经相依赖性方式进行调节。

DOI：

10.1016/s0002-9440(10)65712-4

发表时间：

1998

期刊：

The American journal of pathology

影响因子：

通讯作者：

中科院分区：

文献类型：

作者：

Masaaki Tanaka;Satoru Kyo;M. Takakura;T. Kanaya;Tetsuya Sagawa;Kaname Yamashita;Yasunori Okada;E. Hiyama;M. Inoue

在大多数恶性肿瘤和生殖细胞中观察到端粒酶活性，而正常体细胞通常不表达端粒酶。人子宫内膜由腺和间质成分组成，在月经周期中增殖活性发生剧烈变化，受雌激素功能的精细调节。我们以前报道过正常的人子宫内膜表达端粒酶活性。然而，目前尚不清楚上述哪些成分是端粒酶活性的主要来源，以及端粒酶活性水平如何在月经周期中受到调节。端粒酶活性的定量分析表明，它在月经周期的过程中发生了巨大的变化，并以月经相依赖的方式严格调节。与子宫内膜癌相当的最大活性出现在增殖晚期，最低活性出现在分泌晚期。绝经后子宫内膜和抗雌激素药物治疗的子宫内膜显示端粒酶活性降低。检测分离的上皮腺细胞和基质细胞，我们发现端粒酶活性局限于上皮腺细胞。原位RNA杂交分析也显示了人端粒酶RNA的上皮特异性表达。体外培养的上皮细胞分析表明，端粒酶活性与上皮细胞增殖相关，但不受雌激素治疗的影响。这些发现表明端粒酶活性的表达是上皮细胞特异性的，并与细胞增殖状态有关。雌激素在端粒酶调节中的作用仍有待阐明。

Telomerase activity is observed in most malignant tumors and germ cells, whereas normal somatic cells usually do not express it. Human endometrium is composed of glandular and stromal components and exhibits dramatic changes in proliferative activity during the menstrual cycle, which is exquisitely regulated by estrogen function. We previously reported that normal human endometrium expresses telomerase activity. However, it remains unclear which of the above components are the major sources of telomerase activity and how levels of telomerase activity are regulated over the menstrual cycle. Quantitative analysis of telomerase activity revealed that it changes dramatically over the course of the menstrual cycle and is strictly regulated in a menstrual-phase-dependent manner. Maximal activity equivalent to that in endometrial cancer was present in late proliferative phase, and minimal activity in late secretory phase. Postmenopausal endometrium and endometrium treated with anti-estrogen drugs exhibited decreased telomerase activity. Testing isolated epithelial glandular cells and stromal cells, we found that telomerase activity was localized to epithelial glandular cells.In situ RNA hybridization analysis also revealed epithelial-specific expression of human telomerase RNA. In vitro analysis of cultured epithelial cells demonstrated that telomerase activity is correlated with epithelial proliferation but not affected by estrogen treatment. These findings suggest that expression of telomerase activity is specific to epithelial cells and linked to cell proliferative status. The involvement of estrogen in telomerase regulation remains to be elucidated.