Investigating the biological response of human mesenchymal stem cells to titanium surfaces.
Investigating the biological response of human mesenchymal stem cells to titanium surfaces.
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DOI：
10.1186/s13018-014-0135-y
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发表时间：
2014-12-12
影响因子：
2.6
通讯作者：
Oldershaw RA
中科院分区：
文献类型：
--
作者：
German MJ;Osei-Bempong C;Knuth CA;Deehan DJ;Oldershaw RA
我们研究了一个新的特点人口的关节积血液衍生的人间充质干细胞（HF-hMSCs）与钛（Ti）表面的行为。将HF-hMSC接种到圆形空心干扰（RCI; Smith and Nephew）螺钉或对照钛盘上，并在促成骨条件下培养。电子显微镜显示，在成骨培养的早期阶段，HF-hMSCs在两个钛表面上附着和扩散;然而，细胞仅局限于钛螺钉顶点内的基底区域。在培养的后期阶段，类骨质基质沉积在钛表面上，逐渐培养扩增，基质沉积在钛螺钉的侧面和顶部。细胞含量的定量显示Ti螺钉培养物中的细胞数量显著更高;但是，细胞健康状况没有差异。相反，与钛螺钉相比，钛盘培养物中用于基质钙化定性和定量测量的茜素红染色显著增加。我们的研究结果表明，金属植入物的总地形能够创造微环境壁龛，对细胞行为的影响。这些结果对设计先进的组织工程策略具有意义，这些策略寻求使用细胞材料来增强组织重建后的生物重塑和愈合。
We have investigated the behaviour of a newly characterised population of haemarthrosis fluid-derived human mesenchymal stem cells (HF-hMSCs) with titanium (Ti) surfaces. HF-hMSCs were seeded onto round cannulated interference (RCI; Smith and Nephew) screws or control Ti discs and cultured under pro-osteogenic conditions. Electron microscopy showed the attachment and spreading of HF-hMSCs across both Ti surfaces during the early stages of osteogenic culture; however, cells were exclusively localised to the basal regions within the vertex of the Ti screws. In the later stages of culture, an osteoid matrix was deposited on the Ti surfaces with progressive culture expansion and matrix deposition up the sides and the top of the Ti Screws. Quantification of cellular content revealed a significantly higher number of cells within the Ti screw cultures; however, there was no difference in the cellular health. Conversely, alizarin red staining used as both a qualitative and quantitative measure of matrix calcification was significantly increased in Ti disc cultures compared to those of Ti screws. Our results suggest that the gross topography of the metal implant is able to create microenvironment niches that have an influence on cellular behaviour. These results have implications for the design of advanced tissue engineering strategies that seek to use cellular material to enhance biological remodelling and healing following tissue reconstruction.
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