Time-Resolved Transcriptional Profiling of Epithelial Cells Infected by Intracellular Acinetobacter baumannii.
Time-Resolved Transcriptional Profiling of Epithelial Cells Infected by Intracellular Acinetobacter baumannii.
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DOI：
10.3390/microorganisms9020354
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发表时间：
2021-02-11
期刊：
影响因子：
4.5
通讯作者：
Torrent Burgas M
中科院分区：
文献类型：
--
作者：
Crua Asensio N;Macho Rendón J;Torrent Burgas M
抗药性细菌数量的增加已成为对健康的严重威胁，因此识别、表征和优化新分子以帮助我们克服它们引起的感染变得非常重要。众所周知，鲍氏不动杆菌具有显著的逃避抗菌药物作用的能力，导致其成为引起医院和社区获得性感染的细菌之一。尽管如此，这种病原体如何感染宿主细胞并在宿主细胞内存活尚不清楚。在这项研究中，我们分析了A感染后在人类上皮HeLa细胞中观察到的时间分辨转录谱变化。鲍曼不动杆菌，证明了它如何在宿主细胞中存活并在感染后4小时开始复制。这些发现是通过对RNA进行测序来获得一组差异表达基因（DEG）来了解细菌如何改变宿主细胞的环境以获得自身利益。我们还确定了在这组基因中观察到的共同特征，并确定了揭示高度相互作用的蛋白质的蛋白质-蛋白质网络。这些发现的结合为发现用于治疗多重耐药细菌的新的抗菌候选物铺平了道路。
The rise in the number of antibiotic-resistant bacteria has become a serious threat to health, making it important to identify, characterize and optimize new molecules to help us to overcome the infections they cause. It is well known that Acinetobacter baumannii has a significant capacity to evade the actions of antibacterial drugs, leading to its emergence as one of the bacteria responsible for hospital and community-acquired infections. Nonetheless, how this pathogen infects and survives inside the host cell is unclear. In this study, we analyze the time-resolved transcriptional profile changes observed in human epithelial HeLa cells after infection by A. baumannii, demonstrating how it survives in host cells and starts to replicate 4 h post infection. These findings were achieved by sequencing RNA to obtain a set of Differentially Expressed Genes (DEGs) to understand how bacteria alter the host cells’ environment for their own benefit. We also determine common features observed in this set of genes and identify the protein–protein networks that reveal highly-interacted proteins. The combination of these findings paves the way for the discovery of new antimicrobial candidates for the treatment of multidrug-resistant bacteria.
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影响因子：
16.6
作者：
Crua Asensio N;Muñoz Giner E;de Groot NS;Torrent Burgas M
通讯作者：
Torrent Burgas M
影响因子：
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通讯作者：
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