Odorant Response Properties of Convergent Olfactory Receptor Neurons
Odorant Response Properties of Convergent Olfactory Receptor Neurons
复制标题
会聚嗅觉受体神经元的气味反应特性
DOI：
10.1523/jneurosci.18-12-04560.1998
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
J. Kauer
中科院分区：
文献类型：
--
作者：
T. Bozza;J. Kauer
气味刺激的信息被认为是通过嗅上皮和嗅球（OB）中神经元活动的空间和时间模式来表示的。以往的研究表明，分布在鼻腔的嗅觉受体神经元（ORN）投射到OB肾小球层的局部区域。然而，这种收敛的功能意义尚不清楚，并且在任何研究中都没有直接测量投射到定义OB区域的单个ORN的气味响应特性。我们已经逆行标记了小鼠ORN连接到不同的肾小球在背OB和测试单细胞的反应，气味使用Fura-2钙成像。投射到背内侧（DM）肾小球簇的ORN表现出与投射到背外侧（DL）肾小球的ORN不同的气味反应谱。DL-投射的ORN对具有广泛不同结构的化合物（包括香芹酮、丁香酚、肉桂醛和苯乙酮）表现出反应。相比之下，DM投射神经元表现出更结构限制的一组化合物的反应，并优先响应有机酸。这些数据表明，ORN传入隔离的气味反应和ORN和肾小球输入的同质性不同OB区域。数据还表明，投射到DM肾小球的ORN亚群在功能上是相似的。
Information about odorant stimuli is thought to be represented in spatial and temporal patterns of activity across neurons in the olfactory epithelium and the olfactory bulb (OB). Previous studies suggest that olfactory receptor neurons (ORNs) distributed in the nasal cavity project to localized regions in the glomerular layer of the OB. However, the functional significance of this convergence is not yet known, and in no studies have the odorant response properties of individual ORNs projecting to defined OB regions been measured directly. We have retrogradely labeled mouse ORNs connecting to different glomeruli in the dorsal OB and tested single cells for responses to odorants using fura-2 calcium imaging. ORNs that project to clusters of dorsomedial (DM) glomeruli exhibit different odorant response profiles from those that project to dorsolateral (DL) glomeruli. DL-projecting ORNs showed responses to compounds with widely different structures, including carvone, eugenol, cinnamaldehyde, and acetophenone. In contrast, DM-projecting neurons exhibited responses to a more structurally restricted set of compounds and responded preferentially to organic acids. These data demonstrate that ORN afferents segregate by odorant responsiveness and that the homogeneity of ORN and glomerular input varies with different OB regions. The data also demonstrate that a subpopulation of ORNs projecting to DM glomeruli is functionally similar.
DOI：
10.1073/pnas.90.8.3329
发表时间：
1993-04-15
影响因子：
11.1
作者：
GUTHRIE, KM;ANDERSON, AJ;GALL, C
通讯作者：
GALL, C
大鼠粘膜固有的活动模式：来自电压敏感染料的证据。
影响因子：
2.5
作者：
Youngentob,SL;Kent,PF;Sheehe,PR;Schwob,JE;Tzoumaka,E
通讯作者：
Tzoumaka,E
影响因子：
56.9
作者：
RESTREPO, D;MIYAMOTO, T;TEETER, JH
通讯作者：
TEETER, JH