Minimizing inhibitors during pretreatment while maximizing sugar production in enzymatic hydrolysis through a two-stage hydrothermal pretreatment
Minimizing inhibitors during pretreatment while maximizing sugar production in enzymatic hydrolysis through a two-stage hydrothermal pretreatment
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通过两阶段水热预处理，最大限度地减少预处理过程中的抑制剂，同时最大限度地提高酶水解中的糖产量
DOI：
10.1007/s10570-015-0552-z
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发表时间：
2015-01
期刊：
影响因子：
5.7
通讯作者：
Yong Qiang
中科院分区：
文献类型：
--
作者：
Min Dou-Yong;Xu Rui-sen;Hou Zhe;Lv Jia-qing;Huang Cao-xing;Jin Yong-can;Yong Qiang
水热预处理以其绿色、工艺简单等特点在生化转化过程中具有很好的应用前景。然而，关于其对抑制剂产生的影响的有用数据很少，所述抑制剂延迟随后的酶水解。在这项调查中，三个两阶段的水热预处理与不同的严重程度相结合的物理精炼（PFI磨打浆）进行小麦秸秆，然后酶水解，以评估其对糖回收率和抑制剂的产生的影响。将第一阶段（120 °C，30 min）和第二阶段（150 °C，20 min）组合的预处理在本研究中定义为预处理II。预处理II处理的样品总糖回收率最高，为66%，而抑制剂的生成量相对较低。然而，通过定义为预处理I的较不严格处理或定义为预处理III的较严格处理处理的样品获得较低的总糖回收率。结果表明，两段水热预处理工艺应采用低温、延长停留时间和高温、缩短停留时间两种方式进行，以减少抑制剂，提高总糖回收率。
Hydrothermal pretreatment has shown a great promise in biochemical conversion process improving sugar recovery of enzymatic hydrolysis due to its green and simple process features. However, there is very a little useful data on its impact on the generation of inhibitors retarding the subsequent enzymatic hydrolysis. In this investigation, three two-stage hydrothermal pretreatments with different severities combined with physical refining (PFI mill beating) were performed on wheat straw followed by enzymatic hydrolysis to evaluate its impact on sugar recovery and inhibitor generation. The pretreatment that was combined with first stage (120 °C, 30 min) and second stage (150 °C, 20 min) was defined as pretreatment II in this study. And the sample treated by pretreatment II achieved the highest total sugars recovery of 66 %, while generated a relative low level of inhibitors. However, the sample that was treated by less severe treatment defined as pretreatment I or more severe treatment defined as pretreatment III obtained lower total sugars recovery. The results suggested that the two-stage hydrothermal pretreatment should be operated in the following way to diminish inhibitors and enhance the total sugar recovery: the first stage should be performed at low temperature with an extended residence time, while the second stage should be completed at high temperature with a short residence time.
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