Association Between Serum IgE Levels and the CTLA4 +49A/G and FCER1B -654C/T Polymorphisms in Korean Children With Asthma.
Association Between Serum IgE Levels and the CTLA4 +49A/G and FCER1B -654C/T Polymorphisms in Korean Children With Asthma.
复制标题
DOI：
10.4168/aair.2010.2.2.127
复制
发表时间：
2010-04
期刊：
影响因子：
--
通讯作者：
Hong SJ
中科院分区：
文献类型：
--
作者：
Oh KY;Kang MJ;Choi WA;Kwon JW;Kim BJ;Yu J;Hong SJ
T细胞在细胞介导的免疫、特应性疾病和哮喘中发挥核心作用。CD 28/细胞毒性T淋巴细胞抗原4（CTLA 4）衍生的信号转导的平衡在T细胞的活化和增加的免疫球蛋白E（IgE）应答中起重要作用。本研究的目的是探讨编码CTLA 4和高亲和力IgE受体1B（FCER 1B）的基因多态性与韩国哮喘儿童血清IgE水平之间的关系。我们招募了238名对照组和742名哮喘儿童。采用PCR-限制性片段长度多态性分析（PCR-RFLP）对CTLA 4 + 49 A/G和FCER 1B-654 C/T多态性进行基因分型。我们观察到CTLA 4 + 49 A/G在对照组、哮喘儿童和特应性哮喘儿童中的分布没有差异。与此相反，CTLA 4 + 49 A/G的GA基因型在特应性哮喘儿童中显著高于非特应性哮喘儿童。此外，哮喘儿童和携带1或2个CTLA 4 + 49 A拷贝的特应性哮喘儿童的log Dp/Df特异性IgE水平显著高于+49 G纯合子儿童。CTLA 4和FCER 1B之间的基因-基因相互作用与Dp/Df特异性IgE水平的对数相关，但与哮喘的发生无关。此外，与Dp/Df（-）哮喘儿童相比，Dp/Df（+）哮喘儿童携带的风险等位基因组合基因型升高。CTLA 4 + 49 A/G多态性可能有助于韩国哮喘儿童IgE的产生，尤其是Dp/Df特异性IgE水平，但不是哮喘的直接发展。此外，具有FCER 1B-654 C/T多态性的Dp/Df特异性IgE水平可能涉及累加效应。
T cells play a central role in cell-mediated immunity, atopic disease, and asthma. The balance of CD28/cytotoxic T-lymphocyte antigen 4 (CTLA4)-derived signal transduction plays an important role in the activation of T cells and an increased immunoglobulin E (IgE) response. The aim of the current study was to investigate the association between polymorphisms in the genes encoding both CTLA4 and the high-affinity IgE receptor 1B (FCER1B) and serum IgE levels in Korean children with asthma. We enrolled 238 controls and 742 children with asthma. The CTLA4 +49A/G and FCER1B -654C/T polymorphisms were genotyped by PCR-restriction fragment length polymorphism analysis. We observed no difference in the distribution of CTLA4 +49A/G among controls, children with asthma, and those with atopic asthma. In contrast, the GA genotype of CTLA4 +49A/G in children with atopic asthma was significantly higher compared to that in those with non-atopic asthma. Moreover, significantly higher log Dp/Df-specific IgE levels were found in children with asthma and those with atopic asthma carrying one or two copies of the CTLA4 +49A versus those homozygous for +49G. Gene-gene interactions between CTLA4 and FCER1B with the heterozygote and homozygote of variant genotypes were associated with the log Dp/Df-specific IgE levels, but not asthma development. In addition, children with Dp/Df (+) asthma carried an elevated combined genotype of risk allele compared to those with Dp/Df (-) asthma. The CTLA4 +49A/G polymorphism may contribute to the production of IgE in Korean children with asthma, especially in Dp/Df-specific IgE levels, but not in the direct development of asthma. In addition, Dp/Df-specific IgE levels with a FCER1B -654C/T polymorphism may involve additive effects.
登录
查看更多内容
DOI：
10.1164/ajrccm.161.3.9903128
发表时间：
2000-03-01
影响因子：
24.7
作者：
Hizawa, N;Yamaguchi, E;Kawakami, Y
通讯作者：
Kawakami, Y
影响因子：
6.1
作者：
Hizawa, N.;Maeda, Y.;Nishimura, M.
通讯作者：
Nishimura, M.
影响因子：
32.4
作者：
Dombrowicz, D;Lin, SQ;Kinet, JP
通讯作者：
Kinet, JP
影响因子：
9.6
作者：
Lee, SY;Lee, YH;In, KH
通讯作者：
In, KH
影响因子：
14.2
作者：
Hofer, MF;Jirapongsananuruk, O;Leung, DYM
通讯作者：
Leung, DYM