The N-glycome of human embryonic stem cells.
The N-glycome of human embryonic stem cells.
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DOI：
10.1186/1471-2121-10-42
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发表时间：
2009-06-02
期刊：
影响因子：
--
通讯作者：
Laine J
中科院分区：
文献类型：
--
作者：
Satomaa T;Heiskanen A;Mikkola M;Olsson C;Blomqvist M;Tiittanen M;Jaatinen T;Aitio O;Olonen A;Helin J;Hiltunen J;Natunen J;Tuuri T;Otonkoski T;Saarinen J;Laine J
复杂的碳水化合物结构，聚糖，是糖蛋白、糖脂和蛋白聚糖的基本成分。虽然包括SSEA和Tra抗原在内的单个聚糖结构已经被用于定义未分化的人胚胎干细胞（hESC），但干细胞聚糖的整个谱仍然未知。我们利用MALDI-TOF质谱和核磁共振谱分析对hESC的天冬酰胺连接糖蛋白聚糖（n -聚糖）及其分化后代进行了全球研究。结构分析采用特异性糖苷酶和质谱碎片分析。结果表明，hESC具有一个特征性的n -糖苷，该n -糖苷由一个恒定部分和一个在hESC分化过程中发生变化的可变部分组成。hesc相关的n -聚糖下调，分化细胞中出现新的结构。先前使用SSEA-1抗体已将小鼠胚胎干细胞与复杂聚焦化联系起来。在本研究中，我们发现复合聚焦化是未分化hESC中最典型的糖基化特征。唾液化复合体型n -聚糖中最丰富的复合体聚焦结构是Lex和H型2触角。hESC的n -聚糖表型反映了其分化阶段。在分化过程中，hesc相关的n -聚糖特征被分化的细胞相关结构所取代。结果表明，可以通过直接分析n -聚糖谱来确定hESC的分化阶段。这些结果为hESC的n -聚糖谱提供了第一个概述，并为未来靶向干细胞聚糖的策略奠定了基础。
Complex carbohydrate structures, glycans, are essential components of glycoproteins, glycolipids, and proteoglycans. While individual glycan structures including the SSEA and Tra antigens are already used to define undifferentiated human embryonic stem cells (hESC), the whole spectrum of stem cell glycans has remained unknown. We undertook a global study of the asparagine-linked glycoprotein glycans (N-glycans) of hESC and their differentiated progeny using MALDI-TOF mass spectrometric and NMR spectroscopic profiling. Structural analyses were performed by specific glycosidase enzymes and mass spectrometric fragmentation analyses. The data demonstrated that hESC have a characteristic N-glycome which consists of both a constant part and a variable part that changes during hESC differentiation. hESC-associated N-glycans were downregulated and new structures emerged in the differentiated cells. Previously mouse embryonic stem cells have been associated with complex fucosylation by use of SSEA-1 antibody. In the present study we found that complex fucosylation was the most characteristic glycosylation feature also in undifferentiated hESC. The most abundant complex fucosylated structures were Lex and H type 2 antennae in sialylated complex-type N-glycans. The N-glycan phenotype of hESC was shown to reflect their differentiation stage. During differentiation, hESC-associated N-glycan features were replaced by differentiated cell-associated structures. The results indicated that hESC differentiation stage can be determined by direct analysis of the N-glycan profile. These results provide the first overview of the N-glycan profile of hESC and form the basis for future strategies to target stem cell glycans.
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影响因子：
3.5
作者：
Abeyta, MJ;Clark, AT;Firpo, MT
通讯作者：
Firpo, MT
影响因子：
20.3
作者：
Bhattacharya, B;Miura, T;Puri, RK
通讯作者：
Puri, RK
影响因子：
5.2
作者：
Heiskanen, Annamari;Satomaa, Tero;Laine, Jarmo
通讯作者：
Laine, Jarmo
影响因子：
4.8
作者：
Aoki, Kazuhiro;Perlman, Mindy;Tiemeyer, Michael
通讯作者：
Tiemeyer, Michael
影响因子：
64.8
作者：
GOOI, HC;FEIZI, T;EVANS, MJ
通讯作者：
EVANS, MJ