Ionizing radiation-induced mutant frequencies increase transiently in male germ cells of older mice.
Ionizing radiation-induced mutant frequencies increase transiently in male germ cells of older mice.
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DOI：
10.1016/j.mrgentox.2012.01.003
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发表时间：
2012-05-15
影响因子：
1.9
通讯作者：
Walter, Christi A.
中科院分区：
文献类型：
--
作者：
Xu, Guogang;McMahan, C. Alex;Hildreth, Kim;Garcia, Rebecca A.;Herbert, Damon C.;Walter, Christi A.
男性生殖系中的自发突变频率随着年龄的增长而增加，从而增加了后代患有遗传疾病的风险。在本研究中，我们调查了年龄对电离辐射诱导的雄性生殖系突变的影响。lacI转基因小鼠在4月龄、15月龄和26月龄时接受电离辐射处理，并在电离辐射处理后15天或49天测定粗线期精母细胞和圆形精子细胞的突变频率。处理后15天收集的细胞是辐射分化生精细胞的衍生物，而49天后收集的细胞是精原干细胞的衍生物。结果表明：（1）未照射老年小鼠睾丸生精细胞自发突变频率增加（26月龄），尤其是在圆形精子细胞; 2）突变频率显着增加，圆形精子细胞从中年小鼠（15月龄）和老年小鼠（26月龄）在照射后15和49天与假治疗的老年小鼠相比; 3）15或26月龄小鼠粗线期精母细胞在照射后15天显示突变频率显著增加。这项研究表明，年龄调节电离辐射在男性生殖细胞的诱变反应。
Spontaneous mutant frequency in the male germline increases with age, thereby increasing the risk of siring offspring with genetic disorders. In the present study we investigated the effect of age on ionizing radiation-induced male germline mutagenesis. lacI transgenic mice were treated with ionizing radiation at 4-, 15- and 26-month-old, and mutant frequencies were determined for pachytene spermatocytes and round spermatids at 15 days or 49 days after ionizing radiation treatment. Cells collected 15 days after treatment were derivatives of irradiated differentiating spermatogenic cells while cells collected 49 days later were derivatives of spermatogonial stem cells. The results showed that 1) spontaneous mutant frequency increased in spermatogenic cells recovered from nonirradiated old mice (26-months-old), particularly in the round spermatids; 2) mutant frequencies were significantly increased in round spermatids obtained from middle-aged mice (15-months-old) and old age mice (26-momth-old) at 15 and 49 days after irradiation compared to the sham-treated old mice; and 3) pachytene spermatocytes obtained from 15- or 26-month-old mice displayed a significantly increased mutant frequency at 15 days post irradiation. This study indicates that age modulates the mutagenic response to ionizing radiation in the male germline.
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影响因子：
3.6
作者：
Allen D;Herbert DC;McMahan CA;Rotrekl V;Sobol RW;Wilson SH;Walter CA
通讯作者：
Walter CA
影响因子：
2.4
作者：
Jung, A;Schuppe, HC;Schill, WB
通讯作者：
Schill, WB
影响因子：
3.5
作者：
Cardwell, CR;Carson, DJ;Patterson, CC
通讯作者：
Patterson, CC
影响因子：
30.8
作者：
Moloney, DM;Slaney, SF;Wilkie, AOM
通讯作者：
Wilkie, AOM
影响因子：
5.4
作者：
Hemminki, K;Kyyrönen, P;Vaittinen, P
通讯作者：
Vaittinen, P