Systematic optimization of visible light-induced crosslinking conditions of gelatin methacryloyl (GelMA).
Systematic optimization of visible light-induced crosslinking conditions of gelatin methacryloyl (GelMA).
复制标题
系统优化了甲基丙烯酰明胶（GelMA）的可见光交联条件。
DOI：
10.1038/s41598-021-02830-x
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发表时间：
2021-12-02
影响因子：
4.6
通讯作者：
Chodosh J
中科院分区：
文献类型：
--
作者：
Sharifi S;Sharifi H;Akbari A;Chodosh J
甲基丙烯酰明胶（GelMA）是组织工程中应用最广泛的光交联生物聚合物之一。在存在适当的光引发剂的情况下，光活化触发交联过程，这提供了在生理温度下的形状保真度和稳定性。虽然紫外线（UV）已被广泛探索用于光交联，但其应用与源于UV光毒性的许多生物安全问题有关。曙红Y与TEOA和VC的组合是一种生物安全的光引发系统，可以通过可见光而不是紫外线激活，并绕过这些生物安全问题;然而，交联系统需要微调和优化。为了系统地优化交联条件，我们在本文中独立地改变曙红Y [（EY）]、三乙醇胺（TEOA）、乙烯基己内酰胺（VC）、GelMA前体的浓度和交联时间，并评估这些参数对水凝胶性质的影响。我们的数据显示，除了EY（其表现出最佳浓度（约0.05 mM））之外，增加[TEOA]、[VA]、[GelMA]或交联时间改善了水凝胶的机械（拉伸强度/模量和压缩模量）、粘附（搭接剪切强度）、溶胀、生物降解性质。然而，增加交联试剂（[TEOA]、[VA]、[GelMA]）的浓度降低了三维（3D）细胞培养物中的细胞活力。这项研究使我们能够优化交联条件，以改善GelMA水凝胶的性能，并生成具有不同生物医学应用所必需的定义特性的水凝胶库。
Gelatin methacryloyl (GelMA) is one of the most widely used photo-crosslinkable biopolymers in tissue engineering. In in presence of an appropriate photoinitiator, the light activation triggers the crosslinking process, which provides shape fidelity and stability at physiological temperature. Although ultraviolet (UV) has been extensively explored for photo-crosslinking, its application has been linked to numerous biosafety concerns, originated from UV phototoxicity. Eosin Y, in combination with TEOA and VC, is a biosafe photoinitiation system that can be activated via visible light instead of UV and bypasses those biosafety concerns; however, the crosslinking system needs fine-tuning and optimization. In order to systematically optimize the crosslinking conditions, we herein independently varied the concentrations of Eosin Y [(EY)], triethanolamine (TEOA), vinyl caprolactam (VC), GelMA precursor, and crosslinking times and assessed the effect of those parameters on the properties the hydrogel. Our data showed that except EY, which exhibited an optimal concentration (~ 0.05 mM), increasing [TEOA], [VA], [GelMA], or crosslinking time improved mechanical (tensile strength/modulus and compressive modulus), adhesion (lap shear strength), swelling, biodegradation properties of the hydrogel. However, increasing the concentrations of crosslinking reagents ([TEOA], [VA], [GelMA]) reduced cell viability in 3-dimensional (3D) cell culture. This study enabled us to optimize the crosslinking conditions to improve the properties of the GelMA hydrogel and to generate a library of hydrogels with defined properties essential for different biomedical applications.
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影响因子：
3
作者：
MCGRATH, JE
通讯作者：
MCGRATH, JE
影响因子：
6.4
作者：
Gonzalez-Andrades, Miguel;Sharifi, Roholah;Dohlman, Claes H.
通讯作者：
Dohlman, Claes H.
影响因子：
7.4
作者：
Marek, V.;Potey, A.;Parsadaniantz, S. Melik
通讯作者：
Parsadaniantz, S. Melik
影响因子：
6.2
作者：
Elbert, DL;Hubbell, JA
通讯作者：
Hubbell, JA
影响因子：
2.9
作者：
GAROFF, H;ANSORGE, W
通讯作者：
ANSORGE, W