Expression Pattern of Peroxisome Proliferator-Activated Receptors in Rat Hippocampus following Cerebral Ischemia and Reperfusion Injury.
Expression Pattern of Peroxisome Proliferator-Activated Receptors in Rat Hippocampus following Cerebral Ischemia and Reperfusion Injury.
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脑缺血再灌注损伤后大鼠海马过氧化物酶体增殖物激活受体的表达模式
DOI：
10.1155/2012/596394
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发表时间：
2012
期刊：
影响因子：
2.9
通讯作者：
Yang J
中科院分区：
文献类型：
--
作者：
Wang H;Jiang R;He Q;Zhang Y;Zhang Y;Li Y;Zhuang R;Luo Y;Li Y;Wan J;Tang Y;Yu H;Jiang Q;Yang J
本研究旨在观察大鼠全脑缺血再灌注（I/R）损伤后海马过氧化物酶体增殖物激活受体（PPARα、β和γ）表达的时间依赖性变化。雄性Sprague道利（SD）大鼠进行全脑I/R。分别于I/R后30 min ~ 30 d取大鼠海马组织，采用RT-PCR和Western blot方法检测PPARs mRNA和蛋白的表达水平。大鼠海马组织中PPARs mRNA和蛋白的表达水平均显著升高，在缺血再灌注后24 h达到高峰，随后逐渐下降，至30 d接近对照组水平。结果提示，全脑I/R后15 d内海马PPARs mRNA和蛋白表达明显增加。这些发现可能有助于指导PPARs激动剂在脑损伤的实验和临床治疗中的应用。
The present study was designed to investigate the pattern of time-dependent expression of peroxisome proliferator-activated receptors (PPARα, β, and γ) after global cerebral ischemia and reperfusion (I/R) damage in the rat hippocampus. Male Sprague Dawley (SD) rats were subjected to global cerebral I/R. The rat hippocampi were isolated to detect the expression of PPARs mRNA and protein levels at 30 min–30 d after I/R by RT-PCR and Western blot analysis, respectively. The expression levels of PPARs mRNA and protein in the rat hippocampus significantly increased and peaked at 24 h for PPARα and γ (at 48 h for PPARβ) after I/R, then gradually decreased, and finally approached control levels on d 30. The present results suggest that global cerebral I/R can cause obvious increases of hippocampal PPARs mRNA and protein expression within 15 d after I/R. These findings may help to guide the experimental and clinical therapeutic use of PPARs agonists against brain injury.
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影响因子：
5.3
作者：
Ji, Shengbo;Kronenberg, Golo;Endres, Matthias
通讯作者：
Endres, Matthias
影响因子：
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作者：
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DOI：
10.1124/jpet.106.115758
发表时间：
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5.3
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3.3
作者：
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通讯作者：
Cerù, MP