A Zika Vaccine Generated Using the Chimeric Insect-Specific Binjari Virus Platform Protects against Fetal Brain Infection in Pregnant Mice.
A Zika Vaccine Generated Using the Chimeric Insect-Specific Binjari Virus Platform Protects against Fetal Brain Infection in Pregnant Mice.
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DOI：
10.3390/vaccines8030496
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发表时间：
2020-09-02
期刊：
影响因子：
7.8
通讯作者：
Suhrbier A
中科院分区：
文献类型：
--
作者：
Hazlewood JE;Rawle DJ;Tang B;Yan K;Vet LJ;Nakayama E;Hobson-Peters J;Hall RA;Suhrbier A
寨卡病毒（ZIKV）是先天性寨卡综合征（CZS）的病原体，这是一系列可导致终身残疾的出生缺陷。正在开发一系列疫苗，目标人群包括孕妇和育龄妇女。使用最近描述的基于新型昆虫特异性宾贾里病毒（BinJV）的嵌合黄病毒疫苗技术，我们产生了ZIKV疫苗（BinJ/ZIKA-prME），并在本文中说明了其保护免受胎儿脑感染的能力。雌性IFNAR-/-小鼠用无佐剂的BinJ/ZIKA-prME接种一次，交配，并在胚胎第12.5天用ZIKVPRVABC 59攻击。在BinJ/ZIKA-prME疫苗接种的小鼠的母体血液、胎盘或胎头中未检测到感染性ZIKV。当使用更灵敏的qRT PCR方法测量病毒RNA时，获得了类似的结果。BinJ/ZIKA-prME疫苗接种也没有导致登革热病毒感染或疾病的抗体依赖性增强。因此，BinJ/ZIKA-prME成为预防CSZ的潜在候选疫苗。
Zika virus (ZIKV) is the etiological agent of congenital Zika syndrome (CZS), a spectrum of birth defects that can lead to life-long disabilities. A range of vaccines are in development with the target population including pregnant women and women of child-bearing age. Using a recently described chimeric flavivirus vaccine technology based on the novel insect-specific Binjari virus (BinJV), we generated a ZIKV vaccine (BinJ/ZIKA-prME) and illustrate herein its ability to protect against fetal brain infection. Female IFNAR−/− mice were vaccinated once with unadjuvanted BinJ/ZIKA-prME, were mated, and at embryonic day 12.5 were challenged with ZIKVPRVABC59. No infectious ZIKV was detected in maternal blood, placenta, or fetal heads in BinJ/ZIKA-prME-vaccinated mice. A similar result was obtained when the more sensitive qRT PCR methodology was used to measure the viral RNA. BinJ/ZIKA-prME vaccination also did not result in antibody-dependent enhancement of dengue virus infection or disease. BinJ/ZIKA-prME thus emerges as a potential vaccine candidate for the prevention of CSZ.
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DOI：
10.1111/aji.12102
发表时间：
2013-06
期刊：
American journal of reproductive immunology (New York, N.Y. : 1989)
影响因子：
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作者：
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通讯作者：
Bonney EA
影响因子：
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6.4
作者：
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Mattapallil JJ
DOI：
10.1016/s1473-3099(10)70166-3
发表时间：
2010-10
期刊：
The Lancet. Infectious diseases
影响因子：
--
作者：
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通讯作者：
Mosser DM