Arabidopsis FHY3 and FAR1 Function in Age Gating of Leaf Senescence.
Arabidopsis FHY3 and FAR1 Function in Age Gating of Leaf Senescence.
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拟南芥 FHY3 和 FAR1 在叶片衰老年龄门控中的作用
DOI：
10.3389/fpls.2021.770060
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发表时间：
2021
影响因子：
5.6
通讯作者：
Wang H
中科院分区：
文献类型：
--
作者：
Xie Y;Ma M;Liu Y;Wang B;Wei H;Kong D;Wang H
叶片衰老是叶片发育的末期。光和植物激素乙烯在调节叶片衰老方面发挥着重要作用。然而，它们如何在叶片发育过程中协调调节叶片衰老仍不清楚。在这项研究中，我们发现FHY3和FAR1是光敏色素A介导的光信号所必需的两种同源蛋白，它们与EIN3（乙烯信号传导所必需的关键转录因子）和PIF5（负调节光信号的bHLH转录因子）的DNA结合活性发生物理相互作用并抑制它们的DNA结合活性，并干扰它们的DNA与ORE1启动子的结合，ORE1编码促进叶片衰老的关键NAC转录因子。此外，我们还发现 FHY3、PIF5 和 EIN3 形成三蛋白复合物，并且它们协调调节叶片衰老的进程。我们发现，在衰老或黑暗条件下，FHY3 蛋白的积累减少，从而解除其对 EIN3 和 PIF5 DNA 结合的抑制，导致 ORE1 表达增加和叶片衰老的开始。我们的综合结果表明，FHY3 和 FAR1 在年龄门控机制中发挥作用，通过将光和乙烯信号传导与发育衰老相结合来防止叶片早衰。
Leaf senescence is the terminal stage of leaf development. Both light and the plant hormone ethylene play important roles in regulating leaf senescence. However, how they coordinately regulate leaf senescence during leaf development remains largely unclear. In this study, we show that FHY3 and FAR1, two homologous proteins essential for phytochrome A-mediated light signaling, physically interact with and repress the DNA binding activity of EIN3 (a key transcription factor essential for ethylene signaling) and PIF5 (a bHLH transcription factor negatively regulating light signaling), and interfere with their DNA binding to the promoter of ORE1, which encodes a key NAC transcription factor promoting leaf senescence. In addition, we show that FHY3, PIF5, and EIN3 form a tri-protein complex(es) and that they coordinately regulate the progression of leaf senescence. We show that during aging or under dark conditions, accumulation of FHY3 protein decreases, thus lifting its repression on DNA binding of EIN3 and PIF5, leading to the increase of ORE1 expression and onset of leaf senescence. Our combined results suggest that FHY3 and FAR1 act in an age gating mechanism to prevent precocious leaf senescence by integrating light and ethylene signaling with developmental aging.
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