Can phlorotannins purified extracts constitute a novel pharmacological alternative for microbial infections with associated inflammatory conditions?
Can phlorotannins purified extracts constitute a novel pharmacological alternative for microbial infections with associated inflammatory conditions?
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腓诺纯化提取物是否可以构成具有相关炎症条件的微生物感染的新型药理学替代方法？
DOI：
10.1371/journal.pone.0031145
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Valentão P
中科院分区：
文献类型：
--
作者：
Lopes G;Sousa C;Silva LR;Pinto E;Andrade PB;Bernardo J;Mouga T;Valentão P
细菌和真菌感染以及新出现的多药耐药性正在推动人们对用天然产品对抗这些微生物的兴趣，这些天然产品通常被认为是药物治疗的补充。根皮单宁是一种仅限于棕色海藻的多酚类物质，因其生物学能力而受到认可。本研究首次对从西方葡萄牙海岸的10种优势褐藻中提取的根皮单宁的抗菌、抗真菌、抗炎和抗氧化活性进行了研究。通过特定的二甲氧基苯甲醛（DMBA）方法测定间苯三酚含量，并获得75至969 mg/Kg间苯三酚单位（干物质）的产率。螺旋墨角藻（Fucus spiralis）居首位，其次是3种囊塞藻（Cystoseira）。纯化的提取物的抗炎潜力通过对脂多糖刺激的RAW 264.7巨噬细胞产生一氧化氮（NO）的抑制作用来评估，其中坦氏囊绳（Cystoseira tamarcifolia）是显示出有希望的治疗炎症的活性的巨噬细胞。NO清除能力也在无细胞系统中解决，F。spiralis是能力最强的物种。针对五种革兰氏阳性菌和四种革兰氏阴性菌以及三种真菌菌株检查了提取物的抗菌潜力，这些细菌通常定植在皮肤和粘膜上，并导致食品污染。不同提取物对革兰氏阳性菌更有效，表皮葡萄球菌是最敏感的菌种。在抗真菌活性方面，红色毛癣菌是最敏感的菌种。虽然这些特性的分子机制仍然知之甚少，但所获得的结果使根皮单宁纯化提取物成为治疗广泛的微生物感染的新型和有效的药理学替代品，这些感染通常也存在炎症成分。除了本文所证明的生物学性质之外，也可以优选根皮单宁提取物，以避免通常与合成药物相关的副作用和过敏反应。
Bacterial and fungal infections and the emerging multidrug resistance are driving interest in fighting these microorganisms with natural products, which have generally been considered complementary to pharmacological therapies. Phlorotannins are polyphenols restricted to brown seaweeds, recognized for their biological capacity. This study represents the first research on the antibacterial, antifungal, anti-inflammatory and antioxidant activity of phlorotannins purified extracts, which were obtained from ten dominant brown seaweeds of the occidental Portuguese coast. Phlorotannins content was determined by the specific dimethoxybenzaldehyde (DMBA) method and a yield between 75 and 969 mg/Kg phloroglucinol units (dry matter) was obtained. Fucus spiralis ranked first, followed by three Cystoseira species. The anti-inflammatory potential of the purified extracts was assessed via inhibitory effect on nitric oxide (NO) production by lipopolysaccharide-stimulated RAW 264.7 macrophage cells, Cystoseira tamariscifolia being the one showing promising activity for the treatment of inflammation. NO scavenging ability was also addressed in cell free systems, F. spiralis being the species with highest capacity. The antimicrobial potential of the extracts was checked against five Gram-positive and four Gram-negative bacteria and three fungi strains, that commonly colonize skin and mucosa and are responsible for food contamination. The different extracts were more effective against Gram-positive bacteria, Staphylococcus epidermidis being the most susceptible species. Concerning antifungal activity, Trichophyton rubrum was the most sensitive species. Although the molecular mechanisms underlying these properties remain poorly understood, the results obtained turn phlorotannins purified extracts a novel and potent pharmacological alternative for the treatment of a wide range of microbial infections, which usually also present an inflammatory component. In addition to the biological properties demonstrated herein, phlorotannins extracts may also be preferred, in order to avoid side effects and allergic reactions commonly associated with synthetic drugs.
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影响因子：
3.3
作者：
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通讯作者：
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