Automated multidimensional image analysis reveals a role for Abl in embryonic wound repair
Automated multidimensional image analysis reveals a role for Abl in embryonic wound repair
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自动多维图像分析揭示了 Abl 在胚胎伤口修复中的作用
作者：
Teresa Zulueta;M. Tamada;E. J. Lee;R. Fernandez
胚胎表皮显示出快速修复伤口的非凡能力。胚胎创伤修复是由创伤周围细胞骨架和连接蛋白的进化保守再分布驱动的。果蝇已经成为筛选与伤口闭合有关的因素的模型。然而，遗传筛查受到人工分析方法使用的限制。我们介绍MEDUSA，一种新的图像分析工具，用于自动定量的多细胞和分子动力学的延时共聚焦显微镜数据。我们通过量化果蝇胚胎中的伤口闭合来验证MEDUSA，并且我们表明，我们的自动分析结果与手动描绘和跟踪伤口的分析结果相当，同时显着减少了处理时间。我们证明，MEDUSA也可以应用于三维和四维的细胞行为的调查。使用MEDUSA，我们发现，保守的非受体酪氨酸激酶Abelson（Abl）有助于快速胚胎伤口闭合。我们证明，Abl在胚胎创伤修复过程中，在创伤边缘的丝状肌动蛋白的组织和连接蛋白β-catenin的重新分布中发挥作用。最后，我们讨论了不同的模型中的作用，Abl在调节肌动蛋白的架构和粘附动力学在伤口边缘。
The embryonic epidermis displays a remarkable ability to repair wounds rapidly. Embryonic wound repair is driven by the evolutionary conserved redistribution of cytoskeletal and junctional proteins around the wound. Drosophila has emerged as a model to screen for factors implicated in wound closure. However, genetic screens have been limited by the use of manual analysis methods. We introduce MEDUSA, a novel image-analysis tool for the automated quantification of multicellular and molecular dynamics from time-lapse confocal microscopy data. We validate MEDUSA by quantifying wound closure in Drosophila embryos, and we show that the results of our automated analysis are comparable to analysis by manual delineation and tracking of the wounds, while significantly reducing the processing time. We demonstrate that MEDUSA can also be applied to the investigation of cellular behaviors in three and four dimensions. Using MEDUSA, we find that the conserved nonreceptor tyrosine kinase Abelson (Abl) contributes to rapid embryonic wound closure. We demonstrate that Abl plays a role in the organization of filamentous actin and the redistribution of the junctional protein β-catenin at the wound margin during embryonic wound repair. Finally, we discuss different models for the role of Abl in the regulation of actin architecture and adhesion dynamics at the wound margin.
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影响因子：
2.4
作者：
LONGAKER, MT;WHITBY, DJ;HARRISON, MR
通讯作者：
HARRISON, MR
影响因子：
11.8
作者：
Tamada, Masako;Farrell, Dene L.;Zallen, Jennifer A.
通讯作者：
Zallen, Jennifer A.
影响因子：
3.4
作者：
Bendix, Paul M.;Koenderink, Glisje H.;Weitz, David A.
通讯作者：
Weitz, David A.
DOI：
10.1146/annurev-cellbio-092910-154251
发表时间：
2011
影响因子：
11.3
作者：
Sonnemann KJ;Bement WM
通讯作者：
Bement WM