QTL analysis of the developmental response to L-glutamate in Arabidopsis roots and its genotype-by-environment interactions.
QTL analysis of the developmental response to L-glutamate in Arabidopsis roots and its genotype-by-environment interactions.
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DOI：
10.1093/jxb/erx132
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发表时间：
2017-05-17
影响因子：
6.9
通讯作者：
Forde BG
中科院分区：
文献类型：
--
作者：
Walch-Liu P;Meyer RC;Altmann T;Forde BG
已定位了一个控制拟南芥根发育对谷氨酸敏感性的主效QTL，并确定了其他上位性调节其对环境变化的反应的基因座。拟南芥和其他一些物种的初级根生长已经被证明对外部谷氨酸的存在非常敏感，谷氨酸的信号引起了根构型的重大变化。利用C24和Col-0正反交的两个重组自交系，我们定位了一个大效应数量性状基因座(QTL)GluS1和两个次要QTL GluS2和GluS3，它们共同解释了谷氨酸敏感性的表型变异的41%。利用一组C24/Col-0等基因系证实了3号染色体上GluS1基因座的存在。GluS1被定位在基因At3g44830-At3g46880之间。当在包括温度、遮荫和硝酸盐供应在内的一系列环境条件下重复进行QTL定位时，在控制谷氨酸反应的对照中发现了强烈的基因型与环境的交互作用。在不同的环境条件下，控制这一性状的基因座存在较大差异。在这里，我们提出了在第1和第5染色体上存在上位性控制GluS1基因座对环境温度变化的反应的证据，在20°C到26°C之间。此外，1号染色体长臂上的一个基因座被发现在控制外部硝酸盐信号对抗谷氨酸效应的能力方面发挥着重要作用。我们的结论是，在拟南芥根中存在控制谷氨酸敏感性自然变异的多个基因座，上位性互作在调节谷氨酸敏感性对环境条件变化的响应中起着重要作用。
A major QTL controlling glutamate sensitivity of Arabidopsis root development has been mapped and additional loci identified that epistatically regulate its response to environmental changes. Primary root growth in Arabidopsis and a number of other species has previously been shown to be remarkably sensitive to the presence of external glutamate, with glutamate signalling eliciting major changes in root architecture. Using two recombinant inbred lines from reciprocal crosses between Arabidopsis accessions C24 and Col-0, we have identified one large-effect quantitative trait locus (QTL), GluS1, and two minor QTLs, GluS2 and GluS3, which together accounted for 41% of the phenotypic variance in glutamate sensitivity. The presence of the GluS1 locus on chromosome 3 was confirmed using a set of C24/Col-0 isogenic lines. GluS1 was mapped to an interval between genes At3g44830–At3g46880. When QTL mapping was repeated under a range of environmental conditions, including temperature, shading and nitrate supply, a strong genotype-by-environment interaction in the controls for the glutamate response was identified. Major differences in the loci controlling this trait were found under different environmental conditions. Here we present evidence for the existence of loci on chromosomes 1 and 5 epistatically controlling the response of the GluS1 locus to variations in ambient temperature, between 20°C and 26°C. In addition, a locus on the long arm of chromosome 1 was found to play a major role in controlling the ability of external nitrate signals to antagonize the glutamate effect. We conclude that there are multiple loci controlling natural variation in glutamate sensitivity in Arabidopsis roots and that epistatic interactions play an important role in modulating glutamate sensitivity in response to changes in environmental conditions.
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DOI：
10.1111/tpj.12201
发表时间：
2013-07
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Forde BG;Cutler SR;Zaman N;Krysan PJ
通讯作者：
Krysan PJ
影响因子：
7.3
作者：
Reymond, M;Svistoonoff, S;Desnos, T
通讯作者：
Desnos, T
影响因子：
9.4
作者：
Lei, Lei;Li, Yuan;Ren, Dongtao
通讯作者：
Ren, Dongtao
影响因子：
7.3
作者：
Roy, S. J.;Gilliham, M.;Tester, M.
通讯作者：
Tester, M.
影响因子：
64.8
作者：
Asai, T;Tena, G;Sheen, J
通讯作者：
Sheen, J