Assessment of protein kinase C isozymes by two‐site enzyme immunoassay in human brains and changes in Alzheimer's disease-猫眼课题宝

通过双位点酶联免疫分析评估人脑中蛋白激酶C同工酶及阿尔茨海默病的变化

DOI：

10.1212/wnl.43.7.1407

发表时间：

1993

期刊：

Neurology

影响因子：

9.9

通讯作者：

中科院分区：

医学1区

文献类型：

作者：

S. Shimohama;M. Narita;H. Matsushima;J. Kimura;M. Kameyama;M. Hagiwara;H. Hidaka;T. Taniguchi

我们通过双位点酶免疫测定法定量鉴定α、β和γ型同工酶，评估了正常人和阿尔茨海默病（AD）患者死后脑组织中蛋白激酶C（PKC）的含量。在正常人脑组织中，PKC同工酶以β型为主，各同工酶主要分布于脑组织的膜性部分。AD时，颞叶皮质组织中β型PKC的表达明显减少。AD脑细胞膜组分中α、γ型和胞浆组分中α、β、γ型的含量均低于对照组，但差异无显著性。也有一个显着的降低PKC的水平在AD脑的膜部分，放射性佛波酯结合测定。提示β型PKC同工酶主要存在于人颞叶皮层，β型PKC在膜性部分的水平降低可能反映了与AD发病机制相关的生化缺陷。

We assessed the amount of protein kinase C (PKC) in samples from postmortem normal human and Alzheimer's disease (AD) brains by a two-site enzyme immunoassay that quantitatively identified types α, β, and γ isozymes. In the normal human brain matter, type β was the main type present, the majority of each isozyme of PKC being present in the membranous fraction of the brain tissues. In AD brains, the amount of type β PKC was significantly reduced in the membranous fraction of the temporal cortical tissues. The amounts of types α and γ in the membranous fraction and types α, β, and γ in the cytosolic fraction in AD brains were lower than in the control brains, but the difference was not significant. There was also a significant decrease in the levels of PKC in the membranous fraction of AD brains, as measured by radioactive phorbol ester binding. These results suggest that the type β PKC isozyme is mainly present in the human temporal cortex and that reduced levels of type β PKC in the membranous fraction may reflect a biochemical deficit related specifically to the pathogenesis of AD.