Enhanced vascularization in hybrid PCL/gelatin fibrous scaffolds with sustained release of VEGF.
Enhanced vascularization in hybrid PCL/gelatin fibrous scaffolds with sustained release of VEGF.
复制标题
混合 PCL/明胶纤维支架增强血管化并持续释放 VEGF
DOI：
10.1155/2015/865076
复制
发表时间：
2015
影响因子：
--
通讯作者：
Zhao Q
中科院分区：
文献类型：
--
作者：
Wang K;Chen X;Pan Y;Cui Y;Zhou X;Kong D;Zhao Q
创建一个持久的和功能性的脉管系统是组织工程中最基本的挑战之一。VEGF已被广泛接受为参与血管形成早期阶段的有效血管生成因子。在这项研究中，纤维支架，由PCL和明胶纤维制成。明胶纤维通过肝素固定进一步功能化，为血管内皮生长因子提供结合位点，从而使血管内皮生长因子能够持续释放。体外释放试验证实了VEGF的缓释特性，并且在25天的时间段内观察到稳定释放。体外细胞实验表明，VEGF的释放明显促进了内皮细胞的增殖。更重要的是，体内皮下植入反映了与PCL对应物相比，由于VEGF的持续释放，PCL/明胶支架中的血管化已有效增强。因此，在这项研究中开发的肝素化PCL/明胶支架可能是一个有前途的候选人，为复杂组织的再生与充分的血管化。
Creating a long-lasting and functional vasculature represents one of the most fundamental challenges in tissue engineering. VEGF has been widely accepted as a potent angiogenic factor involved in the early stages of blood vessel formation. In this study, fibrous scaffolds that consist of PCL and gelatin fibers were fabricated. The gelatin fibers were further functionalized by heparin immobilization, which provides binding sites for VEGF and thus enables the sustained release of VEGF. In vitro release test confirms the sustained releasing profile of VEGF, and stable release was observed over a time period of 25 days. In vitro cell assay indicates that VEGF release significantly promoted the proliferation of endothelial cells. More importantly, in vivo subcutaneous implantation reflects that vascularization has been effectively enhanced in the PCL/gelatin scaffolds compared with the PCL counterpart due to the sustained release of VEGF. Therefore, the heparinized PCL/gelatin scaffolds developed in this study may be a promising candidate for regeneration of complex tissues with sufficient vascularization.
登录
查看更多内容
影响因子：
14
作者：
Nillesen, Suzan T. M.;Geutjes, Paul J.;van Kuppevelt, Toin H.
通讯作者：
van Kuppevelt, Toin H.
影响因子：
10.8
作者：
Chung, Yong-Il;Kim, Sang-Ki;Kim, Young Ha
通讯作者：
Kim, Young Ha
影响因子：
--
作者：
Johnson, Peter C.;Mikos, Antonios G.;Jansen, John A.
通讯作者：
Jansen, John A.
影响因子：
14
作者：
Du, Fengyi;Wang, Hao;Zhang, Yuanyuan
通讯作者：
Zhang, Yuanyuan
影响因子：
3.9
作者：
PRESTRELSKI, SJ;FOX, GM;ARAKAWA, T
通讯作者：
ARAKAWA, T