Detection of HTLV-III RNA in lungs of patients with AIDS and pulmonary involvement.
Detection of HTLV-III RNA in lungs of patients with AIDS and pulmonary involvement.
复制标题
艾滋病合并肺部受累患者肺部 HTLV-III RNA 的检测。
DOI：
10.1001/jama.1986.03380170072021
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Robert C. Gallo
中科院分区：
文献类型：
--
作者：
K. J. Chayt;Mary E. Harper;L. Marselle;Edward B. Lewin;Richard M. Rose;J. Oleske;Leon G. Epstein;F. Wong;Robert C. Gallo
大多数患有获得性免疫缺陷综合征（艾滋病）的儿童患者和罕见的成人患者发展为慢性呼吸系统疾病，称为“淋巴细胞间质性肺炎”（LIP）。迄今为止，确定导致这一过程的传染因子的努力失败了。在这项研究中，通过原位分子杂交检测了艾滋病和肺部疾病患者的肺冰冻切片，以检测感染人类t细胞嗜淋巴病毒III型（HTLV-III）并表达病毒RNA的细胞的存在。在患有LIP的婴儿中，发现肺中HTLV-III RNA阳性的细胞频率相对较高（0.1%）。由于本研究中使用的原位杂交技术依赖于HTLV-III基因的表达，并且先前的证据表明，一部分HTLV-III感染的细胞可能不表达病毒RNA，因此这个数字是感染细胞总数的下限。此外，这种程度的肺部感染可能仅限于LIP，因为在10名艾滋病患者和除LIP以外的肺部疾病患者中，只有0%至0.002%的肺细胞病毒RNA表达阳性。因此，HTLV-III可能在感染患者的LIP发展中起直接因果作用，暗示其参与了与该病毒相关的另一种多种临床疾病。
A majority of pediatric patients and rare adult patients with the acquired immunodeficiency syndrome (AIDS) develop a chronic respiratory disorder referred to as "lymphocytic interstitial pneumonitis" (LIP). Efforts to identify an infectious agent responsible for this process so far have failed. In this study, frozen sections of lungs from patients with AIDS and pulmonary disease were tested by in situ molecular hybridization for the presence of cells infected with human T-cell lymphotropic virus type III (HTLV-III) and expressing viral RNA. In the case of an infant with LIP, a relatively high frequency (0.1%) of cells in the lung were found to be positive for HTLV-III RNA. This number is the lower limit of total cells infected since the in situ hybridization technique as applied in this study depends on expression of HTLV-III genes, and previous evidence indicates that a proportion of cells infected with HTLV-III may not express viral RNA. Moreover, this degree of infection of the lung is likely limited to LIP, since in ten patients with AIDS and pulmonary diseases other than LIP, only 0% to 0.002% of cells in lung were positive for viral RNA expression. Thus, HTLV-III may play a direct causal role in the development of LIP in infected patients, implicating its involvement in yet another of the diverse clinical diseases associated with this virus.
影响因子：
56.9
作者：
GONDA, MA;WONGSTAAL, F;GILDEN, RV
通讯作者：
GILDEN, RV
绵羊和山羊的慢病毒病：慢性肺炎、白质脑脊髓炎和关节炎。
DOI：
10.1093/clinids/7.1.89
发表时间：
1985
期刊：
Reviews of infectious diseases
影响因子：
--
作者：
Narayan,O;Cork,LC
通讯作者：
Cork,LC
获得性免疫缺陷综合征的肺部受累。
影响因子：
9.6
作者：
Hopewell,PC;Luce,JM
通讯作者：
Luce,JM