Airway microbiota and pathogen abundance in age-stratified cystic fibrosis patients.
Airway microbiota and pathogen abundance in age-stratified cystic fibrosis patients.
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DOI：
10.1371/journal.pone.0011044
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发表时间：
2010-06-23
期刊：
影响因子：
3.7
通讯作者：
Lynch SV
中科院分区：
文献类型：
--
作者：
Cox MJ;Allgaier M;Taylor B;Baek MS;Huang YJ;Daly RA;Karaoz U;Andersen GL;Brown R;Fujimura KE;Wu B;Tran D;Koff J;Kleinhenz ME;Nielson D;Brodie EL;Lynch SV
囊性纤维化（CF）患者气道中的细菌群落与其他生态位一样，受到自体和同种异体因素的影响。然而，我们对年轻与老年CF气道中的微生物定植以及与肺功能的相关性的了解充其量只是初步的。使用系统发育微阵列，我们检查了年龄分层的CF患者的气道微生物群，范围从新生儿（9个月）到成人（72岁）。从一组临床稳定的患者中，我们证明了肺功能较差的老年CF患者与年轻患者相比具有更不均匀的、遗传学聚集的气道群落。使用从这些患者的子集收集的纵向样本，在年轻患者中观察到初始细菌群落多样化的模式，而在老年患者中随着时间的推移，多样性逐渐丧失。我们详细描述了与年轻和老年CF患者相关的不同细菌群落特征，特别关注各自的“早期”和“晚期”定植生物体之间的差异。最后，我们评估了囊性纤维化跨膜调节因子（CFTR）突变对细菌丰度的影响，并确定了涉及假单胞菌科、黄单胞菌科、莫拉菌科和肠杆菌科等成员的基因型特异性群落。本文提供的数据提供了对CF气道微生物群的见解，包括年轻患者的初始多样化事件以及与老年患者人群肺功能不良相关的病原体专门社区的建立。
Bacterial communities in the airways of cystic fibrosis (CF) patients are, as in other ecological niches, influenced by autogenic and allogenic factors. However, our understanding of microbial colonization in younger versus older CF airways and the association with pulmonary function is rudimentary at best. Using a phylogenetic microarray, we examine the airway microbiota in age stratified CF patients ranging from neonates (9 months) to adults (72 years). From a cohort of clinically stable patients, we demonstrate that older CF patients who exhibit poorer pulmonary function possess more uneven, phylogenetically-clustered airway communities, compared to younger patients. Using longitudinal samples collected form a subset of these patients a pattern of initial bacterial community diversification was observed in younger patients compared with a progressive loss of diversity over time in older patients. We describe in detail the distinct bacterial community profiles associated with young and old CF patients with a particular focus on the differences between respective “early” and “late” colonizing organisms. Finally we assess the influence of Cystic Fibrosis Transmembrane Regulator (CFTR) mutation on bacterial abundance and identify genotype-specific communities involving members of the Pseudomonadaceae, Xanthomonadaceae, Moraxellaceae and Enterobacteriaceae amongst others. Data presented here provides insights into the CF airway microbiota, including initial diversification events in younger patients and establishment of specialized communities of pathogens associated with poor pulmonary function in older patient populations.
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影响因子：
7.6
作者：
Kumar, PS;Griffen, AL;Leys, EJ
通讯作者：
Leys, EJ
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
5.8
作者：
Huber, T;Faulkner, G;Hugenholtz, P
通讯作者：
Hugenholtz, P
DOI：
10.1073/pnas.0608255104
发表时间：
2007-01-02
影响因子：
11.1
作者：
Brodie, Eoin L.;DeSantis, Todd Z.;Andersen, Gary L.
通讯作者：
Andersen, Gary L.
影响因子：
5.8
作者：
Kembel, Steven W.;Cowan, Peter D.;Webb, Campbell O.
通讯作者：
Webb, Campbell O.