Identification of Common Subpopulations of Non-Sorbitol-Fermenting, β-Glucuronidase-Negative Escherichia coli O157:H7 from Bovine Production Environments and Human Clinical Samples
Identification of Common Subpopulations of Non-Sorbitol-Fermenting, β-Glucuronidase-Negative Escherichia coli O157:H7 from Bovine Production Environments and Human Clinical Samples
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从牛生产环境和人类临床样本中鉴定非山梨醇发酵、β-葡萄糖醛酸酶阴性大肠杆菌 O157:H7 的常见亚群
DOI：
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发表时间：
2004
影响因子：
4.4
通讯作者：
A. Benson
中科院分区：
文献类型：
--
作者：
Zhijie Yang;J. Kovar;Jaehyoung Kim;J. Nietfeldt;David R. Smith;R. Moxley;M. E. Olson;P. Fey;A. Benson
摘要 非山梨醇发酵、β-葡萄糖醛酸酶阴性大肠杆菌 O157:H7 菌株被认为是一个克隆复合体，来自不同地理位置的种群被认为拥有一个最近的共同祖先。尽管它们具有相关性，但高分辨率基因分型方法可以检测不同人群之间显着的基因组变异。对这些菌株的高分辨率基因分型数据的系统发育分析表明，来自地理上不相关的大陆的亚群可以分为两个主要的系统发育谱系，称为谱系 I 和谱系 II，对这些谱系分布的有限研究表明，它们在人类中引起疾病或传播给人类的倾向可能存在差异。由于区分两个谱系所需的基因分型方法繁琐且主观，因此这些方法并不特别适合研究系统评估这些谱系的生态和传播特征所需的大量菌株。为了克服这一限制，我们开发了一种谱系特异性多态性测定（LSPA），可以轻松区分谱系 I 和谱系 II 亚群。在此报告的研究中，我们描述了六标记测试 (LSPA-6) 的开发及其与基于八聚体的基因组扫描的并排比较的验证。使用 LSPA-6 对 1,400 多个 O157:H7 菌株进行的分析表明，超过 91% 的菌株有 5 种基因型，表明这些亚群可能广泛存在。
ABSTRACT Non-sorbitol-fermenting, β-glucuronidase-negative Escherichia coli O157:H7 strains are regarded as a clone complex, and populations from different geographical locations are believed to share a recent common ancestor. Despite their relatedness, high-resolution genotyping methods can detect significant genome variation among different populations. Phylogenetic analysis of high-resolution genotyping data from these strains has shown that subpopulations from geographically unlinked continents can be divided into two primary phylogenetic lineages, termed lineage I and lineage II, and limited studies of the distribution of these lineages suggest there could be differences in their propensity to cause disease in humans or to be transmitted to humans. Because the genotyping methods necessary to discriminate the two lineages are tedious and subjective, these methods are not particularly suited for studying the large sets of strains that are required to systematically evaluate the ecology and transmission characteristics of these lineages. To overcome this limitation, we have developed a lineage-specific polymorphism assay (LSPA) that can readily distinguish between the lineage I and lineage II subpopulations. In the studies reported here, we describe the development of a six-marker test (LSPA-6) and its validation in a side-by-side comparison with octamer-based genome scanning. Analysis of over 1,400 O157:H7 strains with the LSPA-6 demonstrated that five genotypes comprise over 91% of the strains, suggesting that these subpopulations may be widespread.
影响因子：
15.9
作者：
DONNENBERG, MS;TZIPORI, S;KAPER, JB
通讯作者：
KAPER, JB
DOI：
10.1073/pnas.92.5.1664
发表时间：
1995-02-28
影响因子：
11.1
作者：
MCDANIEL, TK;JARVIS, KG;KAPER, JB
通讯作者：
KAPER, JB