Characterization of the hypersensitive response-like cell death phenomenon induced by targeting antiviral lectin griffithsin to the secretory pathway.
Characterization of the hypersensitive response-like cell death phenomenon induced by targeting antiviral lectin griffithsin to the secretory pathway.
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DOI：
10.1111/pbi.12917
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发表时间：
2018-10
影响因子：
13.8
通讯作者：
Palmer KE
中科院分区：
文献类型：
--
作者：
Kim BM;Lotter-Stark HCT;Rybicki EP;Chikwamba RK;Palmer KE
Griffithsin (GRFT) is an antiviral lectin, originally derived from a red alga, which is currently being investigated as a topical microbicide to prevent transmission of human immunodeficiency virus (HIV). Targeting GRFT to the apoplast for production in Nicotiana benthamiana resulted in necrotic symptoms associated with a hypersensitive response (HR)‐like cell death, accompanied by H2O2 generation and increased PR1 expression. Mannose‐binding lectins surfactant protein D (SP‐D), cyanovirin‐N (CV‐N) and human mannose‐binding lectin (hMBL) also induce salicylic acid (SA)‐dependent HR‐like cell death in N. benthamiana, and this effect is mediated by the lectin's glycan binding activity. We found that secreted GRFT interacts with an endogenous glycoprotein, α‐xylosidase (XYL1), which is involved in cell wall organization. The necrotic effect could be mitigated by overexpression of Arabidopsis XYL1, and by co‐expression of SA‐degrading enzyme NahG, providing strategies for enhancing expression of oligomannose‐binding lectins in plants.
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影响因子：
4.3
作者：
Günl M;Pauly M
通讯作者：
Pauly M
DOI：
10.1073/pnas.0606631103
发表时间：
2006-10-03
影响因子：
11.1
作者：
Giritch, Anatoli;Marillonnet, Sylvestre;Gleba, Yuri
通讯作者：
Gleba, Yuri
影响因子：
11.6
作者：
Guenl, Markus;Neumetzler, Lutz;Pauly, Markus
通讯作者：
Pauly, Markus
影响因子：
4.8
作者：
Fliegmann, J;Mithöfer, A;Ebel, J
通讯作者：
Ebel, J
影响因子：
56.9
作者：
BROEKAERT, WF;VANPARIJS, J;PEUMANS, WJ
通讯作者：
PEUMANS, WJ