Molecular characterization, activity analysis and transcriptional detection of chitinases encoded in the genome of Spodoptera exigua
Molecular characterization, activity analysis and transcriptional detection of chitinases encoded in the genome of Spodoptera exigua
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甜菜夜蛾基因组编码的几丁质酶的分子表征、活性分析和转录检测
DOI：
10.1016/j.aspen.2019.03.007
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发表时间：
2019-06
影响因子：
1.5
通讯作者：
Guo-Hua Huang
中科院分区：
文献类型：
--
作者：
Lei He;Huan Yu;Cun-Yi Xu;Ying Zhao;Fu-Xiang Yang;Ya-Dong Guo;Guo-Hua Huang
Insect chitinases are necessary hydrolytic enzymes for chitin degradation, insect molting and metamorphosis. In.this study, five chitinases encoded in the genome of Spodoptera exigua (SeCHIT7, SeCHIT11, SeCHIT12, SeCHIT13.and SeCHIT14) were identified by reverse transcription PCR. Phylogenetic analysis indicated that SeCHIT7 belonged to Group III, and SeCHIT11/12/13/14 belonged to Group VIII. Real-time quantitative PCR analyses.showed that SeCHIT7 had an extensive transcription at the fourth- and fifth-instar larval and pupal stages, while.SeCHIT11 had the highest transcription level at the egg stage, and the transcription of SeCHIT12 increased by.over 1000 times from pre-pupal to pupal stage. Tissue-specific analyses showed that these three SeCHITs.(SeCHIT7, SeCHIT11 and SeCHIT12) were mainly transcribed in the midgut and fat body. Chitinase activity.assays suggested that the activity had a lower level at the egg stage and peaked at the pupal stage. The activities.were increased by 9.4 times from egg to first-instar larval stage. Tissue specificity analysis showed that the.highest activity was observed in the fat body, followed by hemolymph and cuticle of the pre-pupal stage..Overall, these results provided valid information for further research on the biological function and regulation of.chitinases in S. exigua.
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影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
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影响因子：
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作者：
Wu, Qingyue;Liu, Tian;Yang, Qing
通讯作者：
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影响因子：
4.6
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Yu H;Li ZQ;He L;Ou-Yang YY;Li N;Huang GH
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Huang GH
DOI：
10.1016/j.bbrc.2006.04.126
发表时间：
2006-06-23
影响因子：
3.1
作者：
Fujita, Kosuke;Shimomura, Kumiko;Suzuki, Koichi
通讯作者：
Suzuki, Koichi
影响因子：
3.8
作者：
Ramalho-Ortigao, JM;Traub-Csekö, YM
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Traub-Csekö, YM