Response analysis of host Spodoptera exigua larvae to infection by Heliothis virescens ascovirus 3h (HvAV-3h) via transcriptome.
Response analysis of host Spodoptera exigua larvae to infection by Heliothis virescens ascovirus 3h (HvAV-3h) via transcriptome.
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通过转录组分析宿主甜菜夜蛾幼虫对烟夜蛾子囊病毒3h（HvAV-3h）感染的反应
DOI：
10.1038/s41598-018-23715-6
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发表时间：
2018-03-29
影响因子：
4.6
通讯作者：
Huang GH
中科院分区：
文献类型：
--
作者：
Yu H;Li ZQ;He L;Ou-Yang YY;Li N;Huang GH
绿色日光夜蛾寄生蜂病毒3h(HvAV-3h)是一种双链DNA昆虫病毒，属于寄生病毒科，可通过产卵寄生寄生蜂来侵染夜蛾科的毛虫。为了全面了解寄主幼虫感染HvAV病毒后的交互反应，对甜菜夜蛾在感染HvAV-3h后6~168h的转录组进行了分析。利用从感染和未感染甜菜夜蛾幼虫中获得的约101.64 GB的RNA测序数据进行从头转录组组装，得到约62,258个甜菜夜蛾非遗传基因。基因差异表达分析表明，大多数寄主转录本从6HPI开始下调，并持续到整个侵染期，尽管Unigene数量在12-72HPI阶段上调。值得注意的是，KEGG注释中最丰富的代谢途径是代谢术语，表明HvAV-3h感染后宿主幼虫的代谢机制受到了很大影响。此外，寄主角质层编码UNIGNES的蛋白在大多数情况下都高度下调，表明寄主幼虫角质层的合成受到病毒感染的抑制。
Heliothis virescens ascovirus 3 h (HvAV-3h), a dsDNA insect virus, belonging to the family Ascoviridae, can infect caterpillars of several Noctuidae species by ovipositing parasitoid wasps. In order to provide a comprehensive overview of the interactive responses of host larvae after infection by the ascovirus, a transcriptome analysis of Spodoptera exigua to HvAV-3h was conducted from 6 to 168 hours post infection (hpi). Approximately 101.64 Gb of RNA sequencing (RNA-seq) data obtained from infected and uninfected S. exigua larvae were used to perform a de novo transcriptome assembly, which generated approximately 62,258 S. exigua unigenes. Using differential gene expression analysis, it was determined that the majority of host transcripts were down-regulated beginning at 6 hpi and continuing throughout the infection period, although there was an increase in up-regulated unigene number during the 12 to 72 hpi stage. It is noteworthy that the most abundantly enriched pathways in KEGG annotation were Metabolism terms, indicating that the host larval metabolic mechanisms were highly influenced post HvAV-3h infection. In addition, the host cuticle protein encoding unigenes were highly down-regulated in most of the situations, suggesting that the host larval cuticle synthesis were inhibited by the viral infection.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
2.6
作者：
Bao, Y-Y.;Lv, Z-Y.;Zhang, C-X.
通讯作者：
Zhang, C-X.
影响因子：
5.4
作者：
Chen, Yun-Ru;Zhong, Silin;Blissard, Gary W.
通讯作者：
Blissard, Gary W.
影响因子：
10.5
作者：
Bideshi, DK;Tan, YP;Federici, BA
通讯作者：
Federici, BA
DOI：
10.1073/pnas.80.24.7664
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
FEDERICI, BA
通讯作者：
FEDERICI, BA