The spatiotemporal program of replication in the genome of Lachancea kluyveri.
The spatiotemporal program of replication in the genome of Lachancea kluyveri.
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DOI：
10.1093/gbe/evt014
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发表时间：
2013
影响因子：
3.3
通讯作者：
Fischer G
中科院分区：
文献类型：
--
作者：
Agier N;Romano OM;Touzain F;Cosentino Lagomarsino M;Fischer G
我们在Lachancea kluyveri的基因组中生成了全基因组复制图谱，并评估了复制与碱基组成之间的关系。该物种在祖先全基因组复制之前从酿酒酵母分化而来。基因组由八条染色体组成，其中一条1Mb的染色体臂的G+C-含量远远高于基因组的其余部分。我们在克鲁维氏乳杆菌中鉴定了252个活跃的复制起始点，并发现在起始点位置上与酿酒酵母和华氏乳杆菌有相当大的差异。尽管酿酒酵母复制的一些全球特征是保守的：着丝粒复制得早，而端粒复制得晚，但我们发现，克鲁维氏链球菌和瓦尔蒂链球菌的复制起点都不是进化中的脆弱部位。在克鲁维氏杆菌中，除了1Mb富含GC的染色体臂外，沿着染色体的复制计时在早期和晚期激活起始区之间交替。这条染色体臂包含一个不同于其他染色体的起源共识基序，并在S期早期复制。我们证明，早熟复制是由于在这条染色体臂上特定缺乏晚激发起始点。此外，我们发现GC含量与距离复制起点的距离之间存在相关性，以及在这个富含GC的染色体臂中，领先链和滞后链之间缺乏与复制相关的组成倾斜。这些发现表明，克鲁维氏乳杆菌基因组中不寻常的碱基组成可能与复制有关。
We generated a genome-wide replication profile in the genome of Lachancea kluyveri and assessed the relationship between replication and base composition. This species diverged from Saccharomyces cerevisiae before the ancestral whole genome duplication. The genome comprises eight chromosomes among which a chromosomal arm of 1 Mb has a G + C-content much higher than the rest of the genome. We identified 252 active replication origins in L. kluyveri and found considerable divergence in origin location with S. cerevisiae and with Lachancea waltii. Although some global features of S. cerevisiae replication are conserved: Centromeres replicate early, whereas telomeres replicate late, we found that replication origins both in L. kluyveri and L. waltii do not behave as evolutionary fragile sites. In L. kluyveri, replication timing along chromosomes alternates between regions of early and late activating origins, except for the 1 Mb GC-rich chromosomal arm. This chromosomal arm contains an origin consensus motif different from other chromosomes and is replicated early during S-phase. We showed that precocious replication results from the specific absence of late firing origins in this chromosomal arm. In addition, we found a correlation between GC-content and distance from replication origins as well as a lack of replication-associated compositional skew between leading and lagging strands specifically in this GC-rich chromosomal arm. These findings suggest that the unusual base composition in the genome of L. kluyveri could be linked to replication.
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DOI：
10.1073/pnas.0304170101
发表时间：
2004-02-10
影响因子：
11.1
作者：
Butler, G;Kenny, C;Wolfe, KH
通讯作者：
Wolfe, KH
影响因子：
3.9
作者：
GU, X;LI, WH
通讯作者：
LI, WH
影响因子：
3.3
作者：
Lang GI;Murray AW
通讯作者：
Murray AW
影响因子：
7
作者：
Di Rienzi SC;Lindstrom KC;Mann T;Noble WS;Raghuraman MK;Brewer BJ
通讯作者：
Brewer BJ
影响因子：
5.6
作者：
Czajkowsky, Daniel M.;Liu, Jie;Shao, Zhifeng
通讯作者：
Shao, Zhifeng