Taking the measure of MicroED.
Taking the measure of MicroED.
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DOI：
10.1016/j.sbi.2017.06.004
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发表时间：
2017-10
影响因子：
6.8
通讯作者：
Gonen T
中科院分区：
文献类型：
--
作者：
Rodriguez JA;Eisenberg DS;Gonen T
现在有可能通过电子冷冻显微镜（cryoEM）常规地确定原子的分辨率结构，这在一定程度上得益于被称为微电子衍射（MicroED）的方法。自2013年首次演示以来，MicroED已经帮助确定了分子量从几百道尔顿到几十万道尔顿不等的各种蛋白质结构。其中一些结构是新的，而另一些则是以前已知的。目前MicroED获得的结构分辨率在3.2 Å ~ 1.0 Å之间，多数优于2.5 Å。不同尺寸和形状、不同空间群对称性和不同溶剂含量的晶体都被MicroED研究过。迄今为止，广泛的晶体探索为纳米晶体的结构测定提供了机会。在这里，我们总结了我们在MicroED的头几年里学到的经验教训，以及我们在由该方法确定的第一个从头开始的结构中所做的尝试。我们重新评估了为MicroED选择合适晶体和从测量数据中提取最有意义的理论考虑。随着世界上越来越多的实验室采用这种技术，我们推测第一个十年MicroED可能会发生什么。
It is now possible to routinely determine atomic resolution structures by electron cryo-microscopy (cryoEM), facilitated in part by the method known as micro electron-diffraction (MicroED). Since its initial demonstration in 2013, MicroED has helped determine a variety of protein structures ranging in molecular weight from a few hundred Daltons to several hundred thousand Daltons. Some of these structures were novel while others were previously known. The resolutions of structures obtained thus far by MicroED range from 3.2 Å to 1.0 Å, with most better than 2.5 Å. Crystals of various sizes and shapes, with different space group symmetries, and with a range of solvent content have all been studied by MicroED. The wide range of crystals explored to date presents the community with a landscape of opportunity for structure determination from nano crystals. Here we summarize the lessons we have learned during the first few years of MicroED, and from our attempts at the first ab initio structure determined by the method. We re-evaluate theoretical considerations in choosing the appropriate crystals for MicroED and for extracting the most meaning out of measured data. With more laboratories worldwide adopting the technique, we speculate what the first decade might hold for MicroED.
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影响因子：
48
作者：
Nannenga, Brent L.;Shi, Dan;Leslie, Andrew G. W.;Gonen, Tamir
通讯作者：
Gonen, Tamir
DOI：
10.1073/pnas.1418733111
发表时间：
2014-12-02
影响因子：
11.1
作者：
Cohen, Aina E.;Soltis, S. Michael;Hodgson, Keith O.
通讯作者：
Hodgson, Keith O.
影响因子：
6.1
作者：
Hattne, Johan;Shi, Dan;Gonen, Tamir
通讯作者：
Gonen, Tamir
DOI：
10.1007/978-1-62703-176-9_9
发表时间：
2013-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Gonen, Tamir
通讯作者：
Gonen, Tamir
影响因子：
48
作者：
Rodriguez, Dayte D.;Grosse, Christian;Uson, Isabel
通讯作者：
Uson, Isabel