Virus-derived sequences from the transcriptomes of two snail vectors of schistosomiasis, Biomphalaria pfeifferi and Bulinus globosus from Kenya.
Virus-derived sequences from the transcriptomes of two snail vectors of schistosomiasis, Biomphalaria pfeifferi and Bulinus globosus from Kenya.
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作者：
Liu S;Zhang SM;Buddenborg SK;Loker ES;Bonning BC
血吸虫病感染人数超过2.3亿，其传播媒介是淡水螺。我们在非洲两种最重要的血吸虫病传播媒介——菲氏双脐螺（BP）和球巴蜗牛（BuG）的转录组中鉴定出了病毒序列。利用Illumina Hi-Seq或454测序技术对26只蜗牛的序列进行测定，然后用Trinity和CAP3软件进行组装，并通过一套生物信息学流程鉴定出假定的病毒序列。利用病毒的RNA依赖的RNA聚合酶和外壳蛋白序列进行系统发育分析，以确定所鉴定出的病毒序列与已知病毒序列之间的亲缘关系。病毒序列是从整个蜗牛全生物群落中鉴定出来的，包括共生体、摄入物质以及与蜗牛被动相关的生物体。我们发现了来自17种以上不同病毒的序列，其中包括5个近乎全长的基因组，这些病毒大多是具有正链RNA基因组的小RNA病毒（即类小核糖核酸病毒），部分可能源自附着或摄入的硅藻。基于系统发育分析，这些病毒中的5种（包括BPV2和BuGV2）以及之前报道的4种光滑双脐螺病毒，与已知的无脊椎动物病毒聚类，被认为是蜗牛的假定病毒。样本中来自其中4种新型病毒的RNA序列的存在已得到证实。鉴定候选蜗牛病毒的基因组序列，为进一步表征包括具有生物医学意义物种在内的腹足纲病毒迈出了第一步。
Schistosomiasis, which infects more than 230 million people, is vectored by freshwater snails. We identified viral sequences in the transcriptomes of Biomphalaria pfeifferi (BP) and Bulinus globosus (BuG), two of the world’s most important schistosomiasis vectors in Africa. Sequences from 26 snails generated using Illumina Hi-Seq or 454 sequencing were assembled using Trinity and CAP3 and putative virus sequences were identified using a bioinformatics pipeline. Phylogenetic analyses were performed using viral RNA-dependent RNA polymerase and coat protein sequences to establish relatedness between virus sequences identified and those of known viruses. Viral sequences were identified from the entire snail holobiont, including symbionts, ingested material and organisms passively associated with the snails. Sequences derived from more than 17 different viruses were found including five near full-length genomes, most of which were small RNA viruses with positive sense RNA genomes (i.e., picorna-like viruses) and some of which are likely derived from adherent or ingested diatoms. Based on phylogenetic analysis, five of these viruses (including BPV2 and BuGV2) along with four Biomphalaria glabrata viruses reported previously, cluster with known invertebrate viruses and are putative viruses of snails. The presence of RNA sequences derived from four of these novel viruses in samples was confirmed. Identification of the genome sequences of candidate snail viruses provides a first step toward characterization of additional gastropod viruses, including from species of biomedical significance.
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影响因子：
16.6
作者：
Adema CM;Hillier LW;Jones CS;Loker ES;Knight M;Minx P;Oliveira G;Raghavan N;Shedlock A;do Amaral LR;Arican-Goktas HD;Assis JG;Baba EH;Baron OL;Bayne CJ;Bickham-Wright U;Biggar KK;Blouin M;Bonning BC;Botka C;Bridger JM;Buckley KM;Buddenborg SK;Lima Caldeira R;Carleton J;Carvalho OS;Castillo MG;Chalmers IW;Christensens M;Clifton S;Cosseau C;Coustau C;Cripps RM;Cuesta-Astroz Y;Cummins SF;di Stephano L;Dinguirard N;Duval D;Emrich S;Feschotte C;Feyereisen R;FitzGerald P;Fronick C;Fulton L;Galinier R;Gava SG;Geusz M;Geyer KK;Giraldo-Calderón GI;de Souza Gomes M;Gordy MA;Gourbal B;Grunau C;Hanington PC;Hoffmann KF;Hughes D;Humphries J;Jackson DJ;Jannotti-Passos LK;de Jesus Jeremias W;Jobling S;Kamel B;Kapusta A;Kaur S;Koene JM;Kohn AB;Lawson D;Lawton SP;Liang D;Limpanont Y;Liu S;Lockyer AE;Lovato TL;Ludolf F;Magrini V;McManus DP;Medina M;Misra M;Mitta G;Mkoji GM;Montague MJ;Montelongo C;Moroz LL;Munoz-Torres MC;Niazi U;Noble LR;Oliveira FS;Pais FS;Papenfuss AT;Peace R;Pena JJ;Pila EA;Quelais T;Raney BJ;Rast JP;Rollinson D;Rosse IC;Rotgans B;Routledge EJ;Ryan KM;Scholte LLS;Storey KB;Swain M;Tennessen JA;Tomlinson C;Trujillo DL;Volpi EV;Walker AJ;Wang T;Wannaporn I;Warren WC;Wu XJ;Yoshino TP;Yusuf M;Zhang SM;Zhao M;Wilson RK
通讯作者：
Wilson RK
影响因子：
3.7
作者：
Boros Á;Pankovics P;Simmonds P;Reuter G
通讯作者：
Reuter G
影响因子：
3.8
作者：
Buddenborg, Sarah K.;Kamel, Bishoy;Loker, Eric S.
通讯作者：
Loker, Eric S.
影响因子：
6.4
作者：
Culley AI;Mueller JA;Belcaid M;Wood-Charlson EM;Poisson G;Steward GF
通讯作者：
Steward GF
影响因子：
5.4
作者：
Kapoor, A.;Victoria, J.;Delwart, E.
通讯作者：
Delwart, E.