Novel positive-sense, single-stranded RNA (+ssRNA) virus with di-cistronic genome from intestinal content of freshwater carp (Cyprinus carpio).
Novel positive-sense, single-stranded RNA (+ssRNA) virus with di-cistronic genome from intestinal content of freshwater carp (Cyprinus carpio).
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DOI：
10.1371/journal.pone.0029145
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Reuter G
中科院分区：
文献类型：
--
作者：
Boros Á;Pankovics P;Simmonds P;Reuter G
在匈牙利维斯普雷姆县的“Lingrinte halastó”鱼池中，通过鱼线钓捕的淡水鲤鱼（Cyprinus carpio）的肠道内容物中，偶然发现了一种具有双顺反子基因组结构的新型正义单链RNA（+ssRNA）病毒（Halastavi árva RNA病毒，HalV; JN 000306）。基因组RNA的完整核苷酸（nt）序列长度为9565 nt，并且包含两个长的非框内开放阅读框（ORF），其被基因间区域分开。ORF 1（复制酶）之前是827 nt的非翻译序列，而ORF 2（衣壳蛋白）之后是139 nt的非翻译区。推导的氨基酸序列与小核糖核酸病毒目病毒科的非结构蛋白（2C类解旋酶、3C类半胱氨酸蛋白酶和3D类RNA依赖性RNA聚合酶）和结构蛋白（VP 2/VP 4/VP 3）的相似性很低（低于32%），与双顺反子病毒的相似性更低。Halastavi árva RNA病毒存在于杂食性淡水鲤鱼的肠道内容物中，但这种病毒的起源和宿主物种仍然未知。独特的病毒序列和实际位置表明，Halastavi árva RNA病毒似乎是新的双顺反子ssRNA病毒的第一个成员。需要进一步研究Halastaviárva RNA病毒在自然界中的特定宿主物种（和谱），生态学和作用。
A novel positive-sense, single-stranded RNA (+ssRNA) virus (Halastavi árva RNA virus, HalV; JN000306) with di-cistronic genome organization was serendipitously identified in intestinal contents of freshwater carps (Cyprinus carpio) fished by line-fishing from fishpond “Lőrinte halastó” located in Veszprém County, Hungary. The complete nucleotide (nt) sequence of the genomic RNA is 9565 nt in length and contains two long - non-in-frame - open reading frames (ORFs), which are separated by an intergenic region. The ORF1 (replicase) is preceded by an untranslated sequence of 827 nt, while an untranslated region of 139 nt follows the ORF2 (capsid proteins). The deduced amino acid (aa) sequences of the ORFs showed only low (less than 32%) and partial similarity to the non-structural (2C-like helicase, 3C-like cystein protease and 3D-like RNA dependent RNA polymerase) and structural proteins (VP2/VP4/VP3) of virus families in Picornavirales especially to members of the viruses with dicistronic genome. Halastavi árva RNA virus is present in intestinal contents of omnivorous freshwater carps but the origin and the host species of this virus remains unknown. The unique viral sequence and the actual position indicate that Halastavi árva RNA virus seems to be the first member of a new di-cistronic ssRNA virus. Further studies are required to investigate the specific host species (and spectrum), ecology and role of Halastavi árva RNA virus in the nature.
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