Enhancing Antibodies' Binding Capacity through Oriented Functionalization of Plasmonic Surfaces.
Enhancing Antibodies' Binding Capacity through Oriented Functionalization of Plasmonic Surfaces.
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DOI：
10.3390/nano11102620
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发表时间：
2021-10-05
期刊：
影响因子：
--
通讯作者：
Malara N
中科院分区：
文献类型：
--
作者：
Coluccio ML;Grillo F;Onesto V;Garo V;Scala C;Cuzzola P;Calfa M;Candeloro P;Gentile F;Piletsky S;Malara N
蛋白A由于其特异性识别免疫球蛋白（IG）的能力而长期用于不同的研究领域。来源于金黄色葡萄球菌的蛋白质通过抗体的Fc区与IG结合，在免疫球蛋白G（IgG）中显示出最强的结合，使其成为纯化和检测中使用最多的蛋白质。本文介绍的研究首次将蛋白A整合到用金纳米颗粒图案化的硅表面上，用于IgG的定向结合。信号检测通过金属增强荧光（MEF）系统传送。定向免疫球蛋白允许暴露片段抗原结合（Fab）区以结合其抗原，从而显著增加每个固定抗体的结合能力。抗体定向在许多诊断设备中至关重要，特别是当任一组分的量有限时。
Protein A has long been used in different research fields due to its ability to specifically recognize immunoglobulins (Ig). The protein derived from Staphylococcus aureus binds Ig through the Fc region of the antibody, showing its strongest binding in immunoglobulin G (IgG), making it the most used protein in its purification and detection. The research presented here integrates, for the first time, protein A to a silicon surface patterned with gold nanoparticles for the oriented binding of IgG. The signal detection is conveyed through a metal enhanced fluorescence (MEF) system. Orienting immunoglobulins allows the exposition of the fragment antigen-binding (Fab) region for the binding to its antigen, substantially increasing the binding capacity per antibody immobilized. Antibodies orientation is of crucial importance in many diagnostics devices, particularly when either component is in limited quantities.
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影响因子：
5.8
作者：
BUIJS, J;LICHTENBELT, JWT;LYKLEMA, J
通讯作者：
LYKLEMA, J
影响因子：
7.4
作者：
Dixit, Chandra Kumar;Vashist, Sandeep Kumar;O'Kennedy, Richard
通讯作者：
O'Kennedy, Richard
影响因子：
14
作者：
Gentile, Francesco;Tirinato, Luca;Decuzzi, Paolo
通讯作者：
Decuzzi, Paolo
影响因子：
4.2
作者：
Candeloro, Patrizio;Grande, Elisabetta;Di Fabrizio, Enzo
通讯作者：
Di Fabrizio, Enzo
影响因子：
4.3
作者：
Gallo V;Lai A;Pasquo A;Almaviva S;Iacobelli S;Persichetti L;Capellini G;Antonini G
通讯作者：
Antonini G