Surface-enhanced Raman scattering (SERS)-based immunosystem for ultrasensitive detection of the 90K biomarker.
Surface-enhanced Raman scattering (SERS)-based immunosystem for ultrasensitive detection of the 90K biomarker.
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DOI：
10.1007/s00216-020-02903-2
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发表时间：
2020-11
影响因子：
4.3
通讯作者：
Antonini G
中科院分区：
文献类型：
--
作者：
Gallo V;Lai A;Pasquo A;Almaviva S;Iacobelli S;Persichetti L;Capellini G;Antonini G
生物体液中肿瘤标志物的研究和个性化是目前支持肿瘤诊断、预后和治疗反应监测的主要工具之一。尽管无症状患者肿瘤标志物的识别对于早期诊断至关重要，但其应用仍受其相对较低的灵敏度和现有方法（如ELISA，质谱）的复杂性的限制。我们开发了一种简单、快速、超灵敏的基于表面增强拉曼散射（SERS）的系统，用于LGALS3BP （90K）生物标志物的检测和定量，该生物标志物被用作模型，基于抗体功能化纳米结构金表面的发展。该检测系统可用于不同生化成分样品的超灵敏检测和表征。总之，与目前用于癌症诊断的方法相比，这项工作可以为开发具有最高预测能力的医疗诊断设备提供基础。本文的在线版本（10.1007/s00216-020-02903-2）包含补充资料，仅供授权用户使用。
The research and the individuation of tumour markers in biological fluids are currently one of the main tools to support diagnosis, prognosis, and monitoring of the therapeutic response in oncology. Although the identification of tumour markers in asymptomatic patients is crucial for early diagnosis, its application is still limited by the relatively low sensitivity and the complexity of existing methods (i.e. ELISA, mass spectrometry). We developed an easy, fast, and ultrasensitive surface-enhanced Raman scattering (SERS)–based system, for the detection and quantitation of the LGALS3BP (90K) biomarker that was used as a model, based on the development of antibody-functionalized nanostructured gold surfaces. The detection system was effective for the ultrasensitive detection and characterization of samples of different biochemical compositions. In conclusion, this work could provide the foundation for the development of a medical diagnostic device with the highest predictive power when compared with the methods currently used in cancer diagnostics. The online version of this article (10.1007/s00216-020-02903-2) contains supplementary material, which is available to authorized users.
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影响因子：
4.6
作者：
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通讯作者：
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影响因子：
1.7
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影响因子：
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通讯作者：
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影响因子：
2.9
作者：
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通讯作者：
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