Human mast cells express stem cell factor
Human mast cells express stem cell factor
复制标题
人类肥大细胞表达干细胞因子
作者：
Shaoli Zhang;David F. Anderson;P. Bradding;W. Coward;Susan M. Baddeley;J. Macleod;James McGill;M. Church;S. Holgate;W. Roche
干细胞因子（SCF）是肥大细胞生长和功能的主要细胞因子调节剂。目前的研究表明，人肥大细胞能够产生SCF。通过RT-PCR在从人肺和皮肤分离的肥大细胞中观察到SCF mRNA的组成型合成。这通过仅类胰蛋白酶（MCT）和类胰蛋白酶/糜蛋白酶（MCTC）亚群的结膜肥大细胞原位杂交得到证实。免疫组化结果显示，SCF蛋白产物主要分布于结膜MCT和MCTC肥大细胞。通过ELISA在分离的肺肥大细胞的培养上清液中检测到可溶性SCF蛋白，并且培养物中肺肥大细胞上的IgE受体（Fcε-RI）的交联不改变SCF mRNA表达或分泌的可溶性SCF蛋白。这与正常受试者和季节性过敏性结膜炎（SAC）患者结膜肥大细胞中SCF mRNA表达水平相似的发现一致。这项研究表明，人类肥大细胞本身是SCF的细胞来源，也是这种生长因子的靶细胞。SCF可能通过旁分泌和自分泌两种机制调节肥大细胞的生长和功能。肥大细胞产生SCF可能通过IgE受体介导途径以外的机制进行调节。© 1998 John Wiley & Sons，Ltd.
Stem cell factor (SCF) is a major cytokine regulator of mast cell growth and function. The present study demonstrates that human mast cells are able to produce SCF. Constitutive synthesis of SCF mRNA was seen in the mast cells isolated from human lung and skin by RT‐PCR. This was confirmed by in situ hybridization in conjunctival mast cells of both tryptase‐only (MCT) and tryptase/chymase (MCTC) subsets. SCF protein product was found in conjunctival MCT and MCTC mast cells by immunohistochemistry. Soluble SCF protein was detected in the culture supernatant of isolated lung mast cells by ELISA, and cross‐linkage of IgE receptor (Fcε–RI) on the lung mast cells in culture did not alter SCF mRNA expression, or the secreted soluble SCF protein. This was consistent with the finding that levels of SCF mRNA expression in conjunctival mast cells were similar between normal subjects and patients with seasonal allergic conjunctivitis (SAC). This study shows that human mast cells themselves are a cellular source of SCF, as well as being target cells for this growth factor. SCF may regulate mast cell growth and function via both paracrine and autocrine mechanisms. The production of SCF by mast cells may be regulated via mechanisms other than IgE receptor‐mediated pathways. © 1998 John Wiley & Sons, Ltd.
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DOI：
10.1073/pnas.88.10.4220
发表时间：
1991-05-01
影响因子：
11.1
作者：
WALSH, LJ;TRINCHIERI, G;MURPHY, GF
通讯作者：
MURPHY, GF
影响因子：
4.4
作者：
Michele Columbo;E. Horowitz;Luis M. Botana;D. MacGlashan;B. Bochner;S. Gillis;Krisztina M. Zsebo;Stephen J. Galli;LAWRENCEM. Lichtenstein
通讯作者：
Michele Columbo;E. Horowitz;Luis M. Botana;D. MacGlashan;B. Bochner;S. Gillis;Krisztina M. Zsebo;Stephen J. Galli;LAWRENCEM. Lichtenstein
影响因子：
20.3
作者：
A. Irani;G. Nilsson;U. Miettinen;S. Craig;L. Ashman;T. Ishizaka;K. Zsebo;L. Schwartz
通讯作者：
A. Irani;G. Nilsson;U. Miettinen;S. Craig;L. Ashman;T. Ishizaka;K. Zsebo;L. Schwartz
DOI：
10.1073/pnas.90.2.735
发表时间：
1993-01
影响因子：
11.1
作者：
H. Mitsui;T. Furitsu;A. Dvorak;A. Irani;L. Schwartz;Naoki Inagaki;M. Takei;K. Ishizaka;K. Zsebo;S. Gillis;T. Ishizaka
通讯作者：
H. Mitsui;T. Furitsu;A. Dvorak;A. Irani;L. Schwartz;Naoki Inagaki;M. Takei;K. Ishizaka;K. Zsebo;S. Gillis;T. Ishizaka
人类嗜碱性粒细胞和肥大细胞在过敏性疾病发病机制中的作用。
DOI：
10.1016/0091-6749(85)90656-6
发表时间：
1985
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Schleimer,RP;Fox,CC;Naclerio,RM;Plaut,M;Creticos,PS;Togias,AG;Warner,JA;Kagey-Sobotka,A;Lichtenstein,LM
通讯作者：
Lichtenstein,LM