Evaluation and optimization of PolyJet 3D-printed materials for cell culture studies.
Evaluation and optimization of PolyJet 3D-printed materials for cell culture studies.
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DOI：
10.1007/s00216-022-03991-y
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发表时间：
2022-05
影响因子：
4.3
通讯作者：
Martin, R. Scott
中科院分区：
文献类型：
--
作者：
Currens, Emily R.;Armbruster, Michael R.;Castiaux, Andre D.;Edwards, James L.;Martin, R. Scott
3D打印在微流体领域的应用是一个快速发展的领域，涉及这些设备中细胞培养的应用也变得令人感兴趣。3D打印可用于创建具有复杂功能并在一个设备中集成不同材料类型的定制设计设备;然而，研究在可用的各种基质上培养细胞的能力的研究较少。这项工作描述了PolyJet 3D打印技术对两种细胞系（牛肺动脉内皮细胞（BPAEC）和Madin-Darby犬肾（MDCK）细胞）在两种不同类型的打印材料（VeroClear或MED 610）上的细胞培养的影响。发现未处理的器械在用于1天或更长时间的研究时，导致培养不成功。研究了各种器械处理方法，最成功的是使用氢氧化钠/偏硅酸钠溶液。经该清洁步骤处理的器械培养出的BPAEC和MDCK细胞与传统培养瓶中获得的细胞更相似（在细胞形态、活力和细胞密度方面）。使用LC-MS/MS分析（通过Orbitrap MS）确定未处理器械的潜在沥滤物。最后，在装置中使用纤维支架用于进一步评价治疗方法，并证明在此类装置中进行3D培养的能力。这项研究将有助于希望在基于PolyJet的3D打印设备中利用这些或其他细胞类型的研究人员。
Use of 3D printing for microfluidics is a rapidly growing area, with applications involving cell culture in these devices also becoming of interest. 3D printing can be used to create custom designed devices that have complex features and integrate different material types in one device; however, there are fewer studies studying the ability to culture cells on the various substrates that are available. This work describes the effect of PolyJet 3D printing technology on cell culture of two cell lines, bovine pulmonary artery endothelial cells (BPAECs) and Madin-Darby Canine Kidney (MDCK) cells, on two different types of printed materials (VeroClear or MED610). It was found that untreated devices, when used for studies of 1 day or more, led to unsuccessful culture. A variety of device treatment methodologies were investigated, with the most success coming from the use of sodium hydroxide/sodium metasilicate solution. Devices treated with this cleaning step resulted in culture of BPAECs and MDCK cells that were more similar to what is obtained in traditional culture flasks (in terms of cell morphology, viability, and cell density). LC-MS/MS analysis (via Orbitrap MS) was used to determine potential leachates from untreated devices. Finally, the use of a fiber scaffold in the devices was utilized to further evaluate the treatment methodology and to also demonstrate the ability to perform 3D culture in such devices. This study will be of use for researchers wanting to utilize these or other cell types in PolyJet-based 3D printed devices.
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3D打印的微流体。
DOI：
10.1002/anie.201504382
发表时间：
2016-03-14
期刊：
Angewandte Chemie (International ed. in English)
影响因子：
--
作者：
Au AK;Huynh W;Horowitz LF;Folch A
通讯作者：
Folch A
DOI：
10.1039/c7ay00756f
发表时间：
2017-06-14
期刊：
Analytical methods : advancing methods and applications
影响因子：
--
作者：
Chen C;Townsend AD;Sell SA;Martin RS
通讯作者：
Martin RS
DOI：
10.1039/d1ay01569a
发表时间：
2021-11-04
期刊：
Analytical methods : advancing methods and applications
影响因子：
--
作者：
Castiaux AD;Selemani MA;Ward MA;Martin RS
通讯作者：
Martin RS
影响因子：
3.9
作者：
Lockwood, Sarah Y.;Erkal, Jayda L.;Spence, Dana M.
通讯作者：
Spence, Dana M.
影响因子：
7.4
作者：
Mahieu NG;Spalding JL;Gelman SJ;Patti GJ
通讯作者：
Patti GJ