Characterisation of the faecal bacterial community in adult and elderly horses fed a high fibre, high oil or high starch diet using 454 pyrosequencing.
Characterisation of the faecal bacterial community in adult and elderly horses fed a high fibre, high oil or high starch diet using 454 pyrosequencing.
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DOI：
10.1371/journal.pone.0087424
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Newbold CJ
中科院分区：
文献类型：
--
作者：
Dougal K;de la Fuente G;Harris PA;Girdwood SE;Pinloche E;Geor RJ;Nielsen BD;Schott HC 2nd;Elzinga S;Newbold CJ
从 17 只动物身上采集粪便样本，每只动物喂食三种不同的饮食（高纤维、高纤维富含淀粉补充剂和高纤维富含油补充剂）。提取 DNA 并对 16SrDNA 的 V1-V2 区域进行 454 焦磷酸测序，以研究马的粪便微生物组。通过比较成熟马（8 匹 5-12 岁的马）和老年马（9 匹 19-28 岁的马），也考虑了年龄的影响。在老年马群中发现多样性减少。在 OTU 水平上发现了饮食之间的显着差异（校正 Q<0.1 时有 52 个 OTU）。发现的大多数差异与硬壁菌门 (37) 有关，其中拟杆菌门 (6)、变形菌门 (3)、放线菌门 (2) 和螺旋菌门 (1) 发生了一些变化。对于纯草料饮食，不添加淀粉或油，我们发现所有马中都存在 30/2934 个 OTU（占序列的 15.9%）。然而，与富含油的补充饮食相关的核心（即存在于所有马中）稍小（25/3029 OTU，10.3％），与富含淀粉的补充饮食相关的核心甚至更小（15/2884 OTU，5.4％）。与所有三种饮食样本相关的核心都非常小（6/5689 OTU 仅占序列的 2.3%），并且以梭菌目为主，其中最丰富的科是 Lachnospiraceae。总之，与单独的饲料相比，基于饲料的饮食加上富含淀粉或油的补充饲料与粪便细菌群落的差异相关。此外，正如在人类中观察到的那样，衰老与细菌多样性的减少有关。然而，这些健康动物的细菌群落结构并没有随着年龄的增长而发生变化。
Faecal samples were collected from seventeen animals, each fed three different diets (high fibre, high fibre with a starch rich supplement and high fibre with an oil rich supplement). DNA was extracted and the V1–V2 regions of 16SrDNA were 454-pyrosequenced to investigate the faecal microbiome of the horse. The effect of age was also considered by comparing mature (8 horses aged 5–12) versus elderly horses (9 horses aged 19–28). A reduction in diversity was found in the elderly horse group. Significant differences between diets were found at an OTU level (52 OTUs at corrected Q<0.1). The majority of differences found were related to the Firmucutes phylum (37) with some changes in Bacteroidetes (6), Proteobacteria (3), Actinobacteria (2) and Spirochaetes (1). For the forage only diet,with no added starch or oil, we found 30/2934 OTUs (accounting for 15.9% of sequences) present in all horses. However the core (i.e. present in all horses) associated with the oil rich supplemented diet was somewhat smaller (25/3029 OTUs, 10.3% ) and the core associated with the starch rich supplemented diet was even smaller (15/2884 OTUs, 5.4% ). The core associated with samples across all three diets was extremely small (6/5689 OTUs accounting for only 2.3% of sequences) and dominated by the order Clostridiales, with the most abundant family being Lachnospiraceae. In conclusion, forage based diets plus starch or oil rich complementary feeds were associated with differences in the faecal bacterial community compared with the forage alone. Further, as observed in people, ageing is associated with a reduction in bacterial diversity. However there was no change in the bacterial community structure in these healthy animals associated with age.
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影响因子：
3.7
作者：
Biagi E;Nylund L;Candela M;Ostan R;Bucci L;Pini E;Nikkïla J;Monti D;Satokari R;Franceschi C;Brigidi P;De Vos W
通讯作者：
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3.7
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影响因子：
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