Through ageing, and beyond: gut microbiota and inflammatory status in seniors and centenarians.
Through ageing, and beyond: gut microbiota and inflammatory status in seniors and centenarians.
复制标题
DOI：
10.1371/journal.pone.0010667
复制
发表时间：
2010-05-17
期刊：
影响因子：
3.7
通讯作者：
De Vos W
中科院分区：
文献类型：
--
作者：
Biagi E;Nylund L;Candela M;Ostan R;Bucci L;Pini E;Nikkïla J;Monti D;Satokari R;Franceschi C;Brigidi P;De Vos W
与年龄相关的胃肠道的生理变化，以及宿主免疫系统的生活方式，营养行为和功能的修改，不可避免地会影响肠道菌群，从而更大的感染易感性。
通过使用人类肠道芯片（Hitchip）和16S rRNA基因的细菌和古细菌的定量PCR，我们探索了年龄相关的年龄相关的肠道菌群组成的差异人类的寿命，生活了100多年。我们观察到，年轻人和七十岁的年轻人的肠道生态系统的微生物组成和多样性高度相似，但与一半的人有很大不同。经过100年与人类宿主的共生关联后，微生物群的特征是富公司人群重新排列，并且兼职厌氧菌的富集，尤其是病原体。百年纪念人中这种受损的微生物群的存在与炎症状态增加（也称为炎症）有关，这是由一系列外周血炎症标记确定的。这可以通过对百岁老人的微生物群进行重塑来解释，粪便中的Prauznitzii和亲戚，据报道的抗炎特性显着下降。作为长寿的标志性细菌，我们确定了特定的limosum菌群和亲戚，在百年纪念中增加了十倍以上。
我们提供了以下事实的证据：衰老过程对人类肠道微生物群的结构及其与宿主的免疫系统的稳态产生了深远的影响。由于其在宿主生理和健康状况中的至关重要的作用，肠道菌群组成的年龄差异可能与老年人群的疾病和脆弱性有关。
Age-related physiological changes in the gastrointestinal tract, as well as modifications in lifestyle, nutritional behaviour, and functionality of the host immune system, inevitably affect the gut microbiota, resulting in a greater susceptibility to infections. By using the Human Intestinal Tract Chip (HITChip) and quantitative PCR of 16S rRNA genes of Bacteria and Archaea, we explored the age-related differences in the gut microbiota composition among young adults, elderly, and centenarians, i.e subjects who reached the extreme limits of the human lifespan, living for over 100 years. We observed that the microbial composition and diversity of the gut ecosystem of young adults and seventy-years old people is highly similar but differs significantly from that of the centenarians. After 100 years of symbiotic association with the human host, the microbiota is characterized by a rearrangement in the Firmicutes population and an enrichment in facultative anaerobes, notably pathobionts. The presence of such a compromised microbiota in the centenarians is associated with an increased inflammatory status, also known as inflammageing, as determined by a range of peripheral blood inflammatory markers. This may be explained by a remodelling of the centenarians' microbiota, with a marked decrease in Faecalibacterium prauznitzii and relatives, symbiotic species with reported anti-inflammatory properties. As signature bacteria of the long life we identified specifically Eubacterium limosum and relatives that were more than ten-fold increased in the centenarians. We provide evidence for the fact that the ageing process deeply affects the structure of the human gut microbiota, as well as its homeostasis with the host's immune system. Because of its crucial role in the host physiology and health status, age-related differences in the gut microbiota composition may be related to the progression of diseases and frailty in the elderly population.
登录
查看更多内容
影响因子：
7.7
作者：
Duncan, BB;Schmidt, MI;Heiss, G
通讯作者：
Heiss, G
影响因子：
3.7
作者：
Claesson MJ;O'Sullivan O;Wang Q;Nikkilä J;Marchesi JR;Smidt H;de Vos WM;Ross RP;O'Toole PW
通讯作者：
O'Toole PW
影响因子：
4.4
作者：
Kaufmann, P;Pfefferkorn, A;Meile, L
通讯作者：
Meile, L
影响因子：
3.7
作者：
Andersson, Anders F.;Lindberg, Mathilda;Jakobsson, Hedvig;Backhed, Fredrik;Nyren, Pal;Engstrand, Lars
通讯作者：
Engstrand, Lars
DOI：
10.1097/mco.0b013e3282f2bfdf
发表时间：
2008-01-01
影响因子：
3.1
作者：
Guigoz, Yves;Dore, Joel;Schiffrina, Eduardo J.
通讯作者：
Schiffrina, Eduardo J.