Expression profiling of Cucumis sativus in response to infection by Pseudoperonospora cubensis.
Expression profiling of Cucumis sativus in response to infection by Pseudoperonospora cubensis.
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DOI：
10.1371/journal.pone.0034954
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Buell CR
中科院分区：
文献类型：
--
作者：
Adhikari BN;Savory EA;Vaillancourt B;Childs KL;Hamilton JP;Day B;Buell CR
卵菌病原体古巴假霜霉(Pseudoperonosporacubensis)是葫芦科霜霉病的病原，目前栽培黄瓜(Cucumis sativus)尚无对该病原体的有效抗性。为了更好地了解宿主对强毒病原体的反应，我们使用全转录组测序在兼容相互作用的整个时间过程中进行了表达谱分析。如本文所述，我们能够检测15,286个黄瓜基因的表达，其中14,476个在从接种后1天(dpi)到8dpi的整个感染过程中表达。在时间点之间的成对比较中，大量基因（1,612 至 3,286 个）存在差异表达。我们观察到关键防御相关基因在 1 dpi 内快速诱导，包括过氧化氢酶、几丁质酶、脂氧合酶、过氧化物酶和蛋白酶抑制剂，表明宿主检测到了病原体。共表达网络分析揭示了具有不同表达模式的转录网络，包括已知防御反应基因在 2 dpi 时的下调，表明病原体对宿主反应的协调抑制。在用拟南芥(Hyaloperonospora arabidopsidis)攻击后，对黄瓜基因表达模式与直系同源拟南芥基因的比较分析揭示了单拷贝直系同源物的相关表达模式，表明这两种双子叶植物宿主对相关病原体具有相似的转录反应。总的来说，本文描述的工作对农业重要病理系统中宿主易感性和病原体毒力之间的相互作用进行了深入分析。
The oomycete pathogen, Pseudoperonospora cubensis, is the causal agent of downy mildew on cucurbits, and at present, no effective resistance to this pathogen is available in cultivated cucumber (Cucumis sativus). To better understand the host response to a virulent pathogen, we performed expression profiling throughout a time course of a compatible interaction using whole transcriptome sequencing. As described herein, we were able to detect the expression of 15,286 cucumber genes, of which 14,476 were expressed throughout the infection process from 1 day post-inoculation (dpi) to 8 dpi. A large number of genes, 1,612 to 3,286, were differentially expressed in pair-wise comparisons between time points. We observed the rapid induction of key defense related genes, including catalases, chitinases, lipoxygenases, peroxidases, and protease inhibitors within 1 dpi, suggesting detection of the pathogen by the host. Co-expression network analyses revealed transcriptional networks with distinct patterns of expression including down-regulation at 2 dpi of known defense response genes suggesting coordinated suppression of host responses by the pathogen. Comparative analyses of cucumber gene expression patterns with that of orthologous Arabidopsis thaliana genes following challenge with Hyaloperonospora arabidopsidis revealed correlated expression patterns of single copy orthologs suggesting that these two dicot hosts have similar transcriptional responses to related pathogens. In total, the work described herein presents an in-depth analysis of the interplay between host susceptibility and pathogen virulence in an agriculturally important pathosystem.
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影响因子：
3.7
作者：
Gaulin E;Madoui MA;Bottin A;Jacquet C;Mathé C;Couloux A;Wincker P;Dumas B
通讯作者：
Dumas B
影响因子：
14.9
作者：
Finn RD;Mistry J;Tate J;Coggill P;Heger A;Pollington JE;Gavin OL;Gunasekaran P;Ceric G;Forslund K;Holm L;Sonnhammer EL;Eddy SR;Bateman A
通讯作者：
Bateman A
影响因子：
3.5
作者：
Huibers, Robin P.;de Jong, Mark;Van den Ackerveken, Guido
通讯作者：
Van den Ackerveken, Guido
影响因子：
2.1
作者：
Irizarry, RA;Hobbs, B;Speed, TP
通讯作者：
Speed, TP
不同性型黄瓜花转录组测序及比较分析
影响因子：
4.4
作者：
Guo, Shaogui;Zheng, Yi;Fei, Zhangjun
通讯作者：
Fei, Zhangjun