Transcriptome of Aphanomyces euteiches: new oomycete putative pathogenicity factors and metabolic pathways.
Transcriptome of Aphanomyces euteiches: new oomycete putative pathogenicity factors and metabolic pathways.
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DOI：
10.1371/journal.pone.0001723
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发表时间：
2008-03-05
期刊：
影响因子：
3.7
通讯作者：
Dumas B
中科院分区：
文献类型：
--
作者：
Gaulin E;Madoui MA;Bottin A;Jacquet C;Mathé C;Couloux A;Wincker P;Dumas B
真丝囊霉是一种引起豆科植物如苜蓿和豌豆的幼苗枯萎病和根腐病的卵菌病原体。隐丝酵母属在遗传学上不同于研究充分的卵菌纲，如疫霉属，含有对植物和水生动物有致病性的物种。首次对A. euteiches，使用从在人工液体培养基中生长或与植物根接触的菌丝体提取的mRNA构建两个cDNA文库。从18，864个高质量的表达序列标签（EST）中获得了7，977个序列的单基因集，并对其潜在功能进行了表征。与卵菌蛋白质组的比较揭示了A. euteiches和那些疫霉属物种，导致鉴定的生物合成途径缺乏疫霉属，新的推定致病性基因和基因家族的扩展编码胞外蛋白，特别是不同类别的蛋白酶。在A. Euteiches是参与粘附的新的胞外蛋白质家族的成员，其包含多达四个类似于真菌纤维素结合结构域的蛋白质结构域。A的比较。真菌序列与完全测序的真核病原体（包括真菌、顶复门和锥虫）的蛋白质组相结合，可以识别A. euteiches基因在这些微生物中具有接近的直系同源物，但在迄今为止测序的其他卵菌中不存在，特别是转运蛋白和非核糖体肽合成酶，并表明存在最初在致病性锥虫中表征的抗氧化应激的防御机制。
Aphanomyces euteiches is an oomycete pathogen that causes seedling blight and root rot of legumes, such as alfalfa and pea. The genus Aphanomyces is phylogenically distinct from well-studied oomycetes such as Phytophthora sp., and contains species pathogenic on plants and aquatic animals. To provide the first foray into gene diversity of A. euteiches, two cDNA libraries were constructed using mRNA extracted from mycelium grown in an artificial liquid medium or in contact to plant roots. A unigene set of 7,977 sequences was obtained from 18,864 high-quality expressed sequenced tags (ESTs) and characterized for potential functions. Comparisons with oomycete proteomes revealed major differences between the gene content of A. euteiches and those of Phytophthora species, leading to the identification of biosynthetic pathways absent in Phytophthora, of new putative pathogenicity genes and of expansion of gene families encoding extracellular proteins, notably different classes of proteases. Among the genes specific of A. euteiches are members of a new family of extracellular proteins putatively involved in adhesion, containing up to four protein domains similar to fungal cellulose binding domains. Comparison of A. euteiches sequences with proteomes of fully sequenced eukaryotic pathogens, including fungi, apicomplexa and trypanosomatids, allowed the identification of A. euteiches genes with close orthologs in these microorganisms but absent in other oomycetes sequenced so far, notably transporters and non-ribosomal peptide synthetases, and suggests the presence of a defense mechanism against oxidative stress which was initially characterized in the pathogenic trypanosomatids.
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影响因子：
3.5
作者：
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通讯作者：
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