Effects of aflatoxin B1 combined with ochratoxin A and/or zearalenone on metabolism, immune function, and antioxidant status in lactating dairy goats.
Effects of aflatoxin B1 combined with ochratoxin A and/or zearalenone on metabolism, immune function, and antioxidant status in lactating dairy goats.
复制标题
DOI：
10.5713/ajas.17.0279
复制
发表时间：
2018-04
期刊：
影响因子：
--
通讯作者：
Cheng J
中科院分区：
文献类型：
--
作者：
Huang S;Zheng N;Fan C;Cheng M;Wang S;Jabar A;Wang J;Cheng J
本试验旨在研究黄曲霉毒素B1 （AFB1）单独或与赭曲霉毒素A （OTA）和/或玉米赤霉烯酮（ZEA）混合使用对奶山羊代谢、免疫功能和抗氧化状态的影响。选取泌乳期崂山奶山羊50只，随机分为5个处理组（n = 10），试验期14 d。分别饲喂50 μg AFB1/kg干物质（DM）（对照）、50 μg AFB1/kg DM+100 μg OTA/kg DM （AFB1+OTA）、50 μg AFB1/kg DM+500 μg ZEA/kg DM （AFB1+ZEA）或50 μg AFB1/kg DM+100 μg OTA/kg DM+500 μg ZEA/kg DM （AFB1+OTA+ZEA）。饲喂AFB1+OTA+ZEA的山羊干物质采食量和产奶量低于对照组。与对照组相比，添加AFB1、OTA和ZEA显著降低了红细胞计数、红细胞压积、平均红细胞体积、平均红细胞血红蛋白和平均血小板体积，显著增加了白细胞计数。与对照组相比，AFB1、OTA和ZEA联合使用显著提高了血清中丙氨酸转氨酶（ALT）和碱性磷酸酶（ALP）活性、总胆红素（TBIL）、白细胞介素-6和丙二醛（MDA）活性，显著降低了血清中免疫球蛋白A浓度、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物（GSH-Px）活性和总抗氧化能力（T-AOC）。与AFB1与ZEA联合施用相比，AFB1与OTA联合施用可导致奶牛ALP、ALT、TBIL和MDA升高，产奶量、SOD和GSH-Px活性以及T-AOC降低。AFB1、OTA和ZEA对奶山羊的毒副作用最大，其他真菌毒素组合的毒副作用程度不同。本研究结果可为预防和处理真菌毒素联合污染动物饲料的后果提供指导。
This experiment investigated the effects of aflatoxin B1 (AFB1) alone or mixed with ochratoxin A (OTA) and/or zearalenone (ZEA) on the metabolism, immune function, and antioxidant status of dairy goats. Fifty lactating Laoshan dairy goats were randomly assigned to one of five treatment groups (n = 10) for 14 days. Goats were fed no additive (control) or administered with 50 μg AFB1/kg dry matter (DM) (AFB1), 50 μg AFB1/kg DM+100 μg OTA/kg DM (AFB1+ OTA), 50 μg AFB1/kg DM+500 μg ZEA/kg DM (AFB1+ZEA), or 50 μg AFB1/kg DM+100 μg OTA/kg DM+500 μg ZEA/kg DM (AFB1+OTA+ZEA). Dry matter intake and milk production were lower in goats fed AFB1+OTA+ZEA than in controls. Supplementation with AFB1, OTA, and ZEA significantly decreased red blood cell count, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, and mean platelet volume, and significantly increased white blood cell count, when compared with the control group. Compared with control, the combination of AFB1, OTA, and ZEA significantly increased alanine aminotransferase (ALT) and alkaline phosphatase (ALP) activities, total bilirubin (TBIL), interleukin-6, and malondialdehyde (MDA), but significantly reduced immunoglobulin A concentration, the activities of superoxide dismutase (SOD) and glutathione peroxides (GSH-Px), and total antioxidant capacity (T-AOC) in serum. Administration of AFB1 combined with OTA led to higher ALP, ALT, TBIL, and MDA, as well as lower milk production, SOD and GSH-Px activities, and T-AOC, than administration of AFB1 combined with ZEA. The mixture of AFB1, OTA, and ZEA exerted the greatest adverse effects on dairy goats, meanwhile the deleterious damage of the other mycotoxin combinations were in varying degrees. The findings of this study could provide guidance for the prevention and treatment of the consequences of contamination of animal feeds with combinations of mycotoxin.
登录
查看更多内容
影响因子：
3.6
作者：
Abeni, F.;Migliorati, L.;Pirlo, G.
通讯作者：
Pirlo, G.
影响因子：
3.5
作者：
Battacone, G;Nudda, A;Pulina, G
通讯作者：
Pulina, G
影响因子：
4.5
作者：
Mikami, O;Yamamoto, S;Nakajima, Y
通讯作者：
Nakajima, Y
影响因子：
4.4
作者：
HUFF, WE;DOERR, JA
通讯作者：
DOERR, JA
影响因子：
0.7
作者：
Ge Baokun;Zhao Kongxiang;Mi Jiebo
通讯作者：
Mi Jiebo