Global fitness profiling of fission yeast deletion strains by barcode sequencing.

Global fitness profiling of fission yeast deletion strains by barcode sequencing.
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DOI:
10.1186/gb-2010-11-6-r60
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发表时间:
2010
期刊:
影响因子:
12.3
通讯作者:
Du LL
Du LL
中科院分区:
生物学1区
文献类型:
--
作者:
Han TX;Xu XY;Zhang MJ;Peng X;Du LL

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全基因组缺失文库是探测基因功能的有力工具,最近已可用于裂殖酵母裂殖酵母。在这里,我们使用深度测序来准确地表征缺失文库中的条形码序列,从而使得能够通过条形码测序来定量测量裂殖酵母缺失菌株的适合度。
A genome-wide deletion library is a powerful tool for probing gene functions and one has recently become available for the fission yeast Schizosaccharomyces pombe. Here we use deep sequencing to accurately characterize the barcode sequences in the deletion library, thus enabling the quantitative measurement of the fitness of fission yeast deletion strains by barcode sequencing.
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